Fluid Flow Dependency in Immunoselective Cell Capture via Liquid Biopsy

Kola Ostrikov, Moein Navvab Kashani, Krasimir Vasilev, Melanie N. Macgregor

Research output: Contribution to journalArticlepeer-review

Abstract

Liquid biopsy targets rare cells that overexpress disease-specific membrane markers and capture these cells via immunoaffinity. The diagnosis efficiency of liquid biopsy can be impaired by the presence of healthy adherent cells also expressing the same biomarkers. Here, we investigated the effect of settling times and rinsing flow rates on the efficiency of EpCAM-based immunocapture using both simulation and experiments with three different cell types. Cell-surface adhesion forces and shear rates were calculated to define the range of rinsing flow rates to test experimentally. Healthy adherent cells did not adhere to blocked immunofunctionalized surfaces within the timeframe of the experiment; however, healthy EpCAM positive cells did bind to the surface to some extent. The greatest difference in capture efficiency was obtained using a high rinsing flow rate of 25 mL/min following 40 min static incubation, indicating that optimizing rinsing flow rates could be a viable option to capture, more specifically, cancer cells overexpressing EpCAM.

Original languageEnglish
Pages (from-to)12388-12396
Number of pages9
JournalLangmuir
Volume37
Issue number42
DOIs
Publication statusPublished - 1 Oct 2021

Bibliographical note

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Keywords

  • Fluid Flow
  • Immuniselective Cell Capture
  • Liquid Biopsy

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