TY - JOUR
T1 - Fluid Flow Dependency in Immunoselective Cell Capture via Liquid Biopsy
AU - Ostrikov, Kola
AU - Kashani, Moein Navvab
AU - Vasilev, Krasimir
AU - Macgregor, Melanie N.
N1 - Publisher Copyright:
©
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Liquid biopsy targets rare cells that overexpress disease-specific membrane markers and capture these cells via immunoaffinity. The diagnosis efficiency of liquid biopsy can be impaired by the presence of healthy adherent cells also expressing the same biomarkers. Here, we investigated the effect of settling times and rinsing flow rates on the efficiency of EpCAM-based immunocapture using both simulation and experiments with three different cell types. Cell-surface adhesion forces and shear rates were calculated to define the range of rinsing flow rates to test experimentally. Healthy adherent cells did not adhere to blocked immunofunctionalized surfaces within the timeframe of the experiment; however, healthy EpCAM positive cells did bind to the surface to some extent. The greatest difference in capture efficiency was obtained using a high rinsing flow rate of 25 mL/min following 40 min static incubation, indicating that optimizing rinsing flow rates could be a viable option to capture, more specifically, cancer cells overexpressing EpCAM.
AB - Liquid biopsy targets rare cells that overexpress disease-specific membrane markers and capture these cells via immunoaffinity. The diagnosis efficiency of liquid biopsy can be impaired by the presence of healthy adherent cells also expressing the same biomarkers. Here, we investigated the effect of settling times and rinsing flow rates on the efficiency of EpCAM-based immunocapture using both simulation and experiments with three different cell types. Cell-surface adhesion forces and shear rates were calculated to define the range of rinsing flow rates to test experimentally. Healthy adherent cells did not adhere to blocked immunofunctionalized surfaces within the timeframe of the experiment; however, healthy EpCAM positive cells did bind to the surface to some extent. The greatest difference in capture efficiency was obtained using a high rinsing flow rate of 25 mL/min following 40 min static incubation, indicating that optimizing rinsing flow rates could be a viable option to capture, more specifically, cancer cells overexpressing EpCAM.
KW - Fluid Flow
KW - Immuniselective Cell Capture
KW - Liquid Biopsy
UR - http://www.scopus.com/inward/record.url?scp=85117074591&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/ARC/DP180101254
UR - http://purl.org/au-research/grants/ARC/FT200100301
UR - http://purl.org/au-research/grants/NHMRC/1194466
U2 - 10.1021/acs.langmuir.1c01998
DO - 10.1021/acs.langmuir.1c01998
M3 - Article
AN - SCOPUS:85117074591
VL - 37
SP - 12388
EP - 12396
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 42
ER -