Fluorescent protein as a tool for investigating meiotic recombination in Neurospora.

David Catcheside, Patricia Yeadon, Frederick Bowring

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    Abstract

    We have built a series of Neurospora crassa strains containing alleles of green fluorescent protein (GFP) to provide a visual phenotype for investigating meiotic recombination. These strains provide a convenient means of screening the Neurospora knockout library for genes involved in genetic recombination. They permit rapid analysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for GFP. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.

    Original languageEnglish
    Title of host publicationMeiosis
    PublisherSpringer
    Pages133-145
    Number of pages13
    ISBN (Electronic)9781493963409
    ISBN (Print)9781493963386
    DOIs
    Publication statusPublished - 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1471
    ISSN (Print)1064-3745

    Keywords

    • Crossing over
    • GFP
    • Meiosis
    • Neurospora
    • Octads
    • Recombination
    • Tetrad analysis

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