FMC-HU-1-B: A lymphoma B-cell line with unusual characteristics

Ram Seshadri; Chris Matthews; Peter B. Jacky; Helen Moore; Heddy Zola; Douglas W. Henderson; Alexander A. Morley

doi:10.1016/0145-2126(85)90024-4

FMC-HU-1-B: A lymphoma B-cell line with unusual characteristics

Ram Seshadri, Chris Matthews, Peter B. Jacky, Helen Moore, Heddy Zola, Douglas W. Henderson, Alexander A. Morley

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A cell line (FMC-Hu-1-B) was established from a biopsy of an abdominal mass of a child with non-Burkitt's lymphoma. The establishment of the cell line initially required the presence of normal bone marrow stromal cells and phytohaemagglutinin stimulated leucocyte conditioned medium. The cell line lacked Epstein-Barr virus nuclear antigen and exhibited numerous chromosomal abnormalities. Cell-surface marker analysis using a panel of monoclonal antibodies revealed only markers of the B lineage. Within the B-cell lineage FMC-Hu-1-B seemed to occupy a level of maturation equivalent to normal mature B-cells (surface membrane IgM, secretion of immunoglobulin and FMC-1 positive). However, the cells also weakly expressed the common acute lymphoblastic leukaemia antigen, normally found on early precursors of B-cells. The cells appear to secrete auto-stimulatory growth factor(s).

Original language	English
Pages (from-to)	97-111
Number of pages	15
Journal	Leukemia Research
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/0145-2126(85)90024-4
Publication status	Published - 1985
Externally published	Yes

Keywords

B-cell lymphoma
cell line
cloning
surface markers

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "FMC-HU-1-B: A lymphoma B-cell line with unusual characteristics",

abstract = "A cell line (FMC-Hu-1-B) was established from a biopsy of an abdominal mass of a child with non-Burkitt's lymphoma. The establishment of the cell line initially required the presence of normal bone marrow stromal cells and phytohaemagglutinin stimulated leucocyte conditioned medium. The cell line lacked Epstein-Barr virus nuclear antigen and exhibited numerous chromosomal abnormalities. Cell-surface marker analysis using a panel of monoclonal antibodies revealed only markers of the B lineage. Within the B-cell lineage FMC-Hu-1-B seemed to occupy a level of maturation equivalent to normal mature B-cells (surface membrane IgM, secretion of immunoglobulin and FMC-1 positive). However, the cells also weakly expressed the common acute lymphoblastic leukaemia antigen, normally found on early precursors of B-cells. The cells appear to secrete auto-stimulatory growth factor(s).",

keywords = "B-cell lymphoma, cell line, cloning, surface markers",

author = "Ram Seshadri and Chris Matthews and Jacky, {Peter B.} and Helen Moore and Heddy Zola and Henderson, {Douglas W.} and Morley, {Alexander A.}",

year = "1985",

doi = "10.1016/0145-2126(85)90024-4",

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T2 - A lymphoma B-cell line with unusual characteristics

AU - Seshadri, Ram

AU - Matthews, Chris

AU - Jacky, Peter B.

AU - Moore, Helen

AU - Zola, Heddy

AU - Henderson, Douglas W.

AU - Morley, Alexander A.

PY - 1985

Y1 - 1985

N2 - A cell line (FMC-Hu-1-B) was established from a biopsy of an abdominal mass of a child with non-Burkitt's lymphoma. The establishment of the cell line initially required the presence of normal bone marrow stromal cells and phytohaemagglutinin stimulated leucocyte conditioned medium. The cell line lacked Epstein-Barr virus nuclear antigen and exhibited numerous chromosomal abnormalities. Cell-surface marker analysis using a panel of monoclonal antibodies revealed only markers of the B lineage. Within the B-cell lineage FMC-Hu-1-B seemed to occupy a level of maturation equivalent to normal mature B-cells (surface membrane IgM, secretion of immunoglobulin and FMC-1 positive). However, the cells also weakly expressed the common acute lymphoblastic leukaemia antigen, normally found on early precursors of B-cells. The cells appear to secrete auto-stimulatory growth factor(s).

AB - A cell line (FMC-Hu-1-B) was established from a biopsy of an abdominal mass of a child with non-Burkitt's lymphoma. The establishment of the cell line initially required the presence of normal bone marrow stromal cells and phytohaemagglutinin stimulated leucocyte conditioned medium. The cell line lacked Epstein-Barr virus nuclear antigen and exhibited numerous chromosomal abnormalities. Cell-surface marker analysis using a panel of monoclonal antibodies revealed only markers of the B lineage. Within the B-cell lineage FMC-Hu-1-B seemed to occupy a level of maturation equivalent to normal mature B-cells (surface membrane IgM, secretion of immunoglobulin and FMC-1 positive). However, the cells also weakly expressed the common acute lymphoblastic leukaemia antigen, normally found on early precursors of B-cells. The cells appear to secrete auto-stimulatory growth factor(s).

KW - B-cell lymphoma

KW - cell line

KW - cloning

KW - surface markers

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JO - Leukemia Research

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ER -

FMC-HU-1-B: A lymphoma B-cell line with unusual characteristics

Abstract

Keywords

UN SDGs

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