Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing

Anastasia Khodakova; Leigh Burgoyne; Damien Abarno; Adrian Linacre

doi:10.1016/j.fsigss.2013.10.019

Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing

Anastasia Khodakova, Leigh Burgoyne, Damien Abarno, Adrian Linacre

Research output: Contribution to conference › Paper › peer-review

5 Citations (Scopus)

Abstract

Soil is a remarkably complex, diverse, ubiquitous, and easily transferred material which can reveal highly useful information to assist forensic investigations. In spite of its potential usefulness, the use of genetic soil analysis appears to be currently underestimated in forensic practice. Herein we report on the use of single arbitrarily primed amplification followed by high throughput sequencing of DNA fragments for the comparison of soil samples. The composition and functional attributes of soil microbial communities from three different locations were compared and shown to be different based on the metagenomic sequencing data obtained.

Original language	English
Pages	e39-e40
DOIs	https://doi.org/10.1016/j.fsigss.2013.10.019
Publication status	Published - 28 Oct 2013
Event	25th World Congress of the International Society for Forensic Genetics - Melbourne, Australia Duration: 2 Sep 2013 → 7 Sep 2013 Conference number: 25

Conference

Conference	25th World Congress of the International Society for Forensic Genetics
Abbreviated title	ISFG 2013
Country/Territory	Australia
City	Melbourne
Period	2/09/13 → 7/09/13

Keywords

Arbitrarily primed PCR
Forensic soil analysis
High throughput sequencing
Soil metagenomics

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10.1016/j.fsigss.2013.10.019

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title = "Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing",

abstract = "Soil is a remarkably complex, diverse, ubiquitous, and easily transferred material which can reveal highly useful information to assist forensic investigations. In spite of its potential usefulness, the use of genetic soil analysis appears to be currently underestimated in forensic practice. Herein we report on the use of single arbitrarily primed amplification followed by high throughput sequencing of DNA fragments for the comparison of soil samples. The composition and functional attributes of soil microbial communities from three different locations were compared and shown to be different based on the metagenomic sequencing data obtained.",

keywords = "Arbitrarily primed PCR, Forensic soil analysis, High throughput sequencing, Soil metagenomics",

author = "Anastasia Khodakova and Leigh Burgoyne and Damien Abarno and Adrian Linacre",

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day = "28",

doi = "10.1016/j.fsigss.2013.10.019",

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Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing. / Khodakova, Anastasia; Burgoyne, Leigh; Abarno, Damien; Linacre, Adrian.

2013. e39-e40 Paper presented at 25th World Congress of the International Society for Forensic Genetics, Melbourne, Victoria, Australia.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing

AU - Khodakova, Anastasia

AU - Burgoyne, Leigh

AU - Abarno, Damien

AU - Linacre, Adrian

N1 - Conference code: 25

PY - 2013/10/28

Y1 - 2013/10/28

N2 - Soil is a remarkably complex, diverse, ubiquitous, and easily transferred material which can reveal highly useful information to assist forensic investigations. In spite of its potential usefulness, the use of genetic soil analysis appears to be currently underestimated in forensic practice. Herein we report on the use of single arbitrarily primed amplification followed by high throughput sequencing of DNA fragments for the comparison of soil samples. The composition and functional attributes of soil microbial communities from three different locations were compared and shown to be different based on the metagenomic sequencing data obtained.

AB - Soil is a remarkably complex, diverse, ubiquitous, and easily transferred material which can reveal highly useful information to assist forensic investigations. In spite of its potential usefulness, the use of genetic soil analysis appears to be currently underestimated in forensic practice. Herein we report on the use of single arbitrarily primed amplification followed by high throughput sequencing of DNA fragments for the comparison of soil samples. The composition and functional attributes of soil microbial communities from three different locations were compared and shown to be different based on the metagenomic sequencing data obtained.

KW - Arbitrarily primed PCR

KW - Forensic soil analysis

KW - High throughput sequencing

KW - Soil metagenomics

UR - http://www.scopus.com/inward/record.url?scp=84889881667&partnerID=8YFLogxK

U2 - 10.1016/j.fsigss.2013.10.019

DO - 10.1016/j.fsigss.2013.10.019

M3 - Paper

SP - e39-e40

T2 - 25th World Congress of the International Society for Forensic Genetics

Y2 - 2 September 2013 through 7 September 2013

ER -

Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing

Abstract

Conference

Keywords

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