Forensic analysis of soils using single arbitrarily primed amplification and high throughput sequencing

Anastasia Khodakova, Leigh Burgoyne, Damien Abarno, Adrian Linacre

    Research output: Contribution to conferencePaperpeer-review

    5 Citations (Scopus)

    Abstract

    Soil is a remarkably complex, diverse, ubiquitous, and easily transferred material which can reveal highly useful information to assist forensic investigations. In spite of its potential usefulness, the use of genetic soil analysis appears to be currently underestimated in forensic practice. Herein we report on the use of single arbitrarily primed amplification followed by high throughput sequencing of DNA fragments for the comparison of soil samples. The composition and functional attributes of soil microbial communities from three different locations were compared and shown to be different based on the metagenomic sequencing data obtained.

    Original languageEnglish
    Pagese39-e40
    DOIs
    Publication statusPublished - 28 Oct 2013
    Event25th World Congress of the International Society for Forensic Genetics - Melbourne, Australia
    Duration: 2 Sep 20137 Sep 2013
    Conference number: 25

    Conference

    Conference25th World Congress of the International Society for Forensic Genetics
    Abbreviated titleISFG 2013
    Country/TerritoryAustralia
    CityMelbourne
    Period2/09/137/09/13

    Keywords

    • Arbitrarily primed PCR
    • Forensic soil analysis
    • High throughput sequencing
    • Soil metagenomics

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