Fragmentation of platymonas subcordiformis in anaerobic environment and effect on hydrogenase activity

Fei Yan; Zhao'an Chen; Hongbin Lu; Xingju Yu; Meifang Jin; Wei Zhang

Fragmentation of platymonas subcordiformis in anaerobic environment and effect on hydrogenase activity

Fei Yan, Zhao'an Chen, Hongbin Lu, Xingju Yu, Meifang Jin, Wei Zhang

Research output: Contribution to journal › Article › peer-review

Abstract

To isolate and purify dissolvable hydrogenase in Platymonas subcordiformis cells, the scheme for alga cell fragmentation is designed in anaerobic environment, and the effects of experimental conditions on the hydrogenase activity are investigated. By means of ultrasonication, the concentration of total protein approaches to 2.73 g/L under the condition of 400 W output power, 5 s crushing time, 10 s interval for 20 times. The efficiency of hydrogenase isolation gets higher in case of glass beads or 0.2%(volume fraction) Triton X-100 to break up fresh cells to obtain 1.44 U/g and 1.27 U/g respectively, while the other proteins get less in crude extract by Triton X-100 method, which is suggested to gain enough purified hydrogenase.

Original language	English
Pages (from-to)	497-500
Number of pages	4
Journal	Hsi-An Chiao Tung Ta Hsueh/Journal of Xi'an Jiaotong University
Volume	42
Issue number	4
Publication status	Published - 1 Apr 2008
Externally published	Yes

Keywords

Cell fragmentation
Hydrogenase activity
Platymonas subcordiformis
Total protein concentration

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title = "Fragmentation of platymonas subcordiformis in anaerobic environment and effect on hydrogenase activity",

abstract = "To isolate and purify dissolvable hydrogenase in Platymonas subcordiformis cells, the scheme for alga cell fragmentation is designed in anaerobic environment, and the effects of experimental conditions on the hydrogenase activity are investigated. By means of ultrasonication, the concentration of total protein approaches to 2.73 g/L under the condition of 400 W output power, 5 s crushing time, 10 s interval for 20 times. The efficiency of hydrogenase isolation gets higher in case of glass beads or 0.2%(volume fraction) Triton X-100 to break up fresh cells to obtain 1.44 U/g and 1.27 U/g respectively, while the other proteins get less in crude extract by Triton X-100 method, which is suggested to gain enough purified hydrogenase.",

keywords = "Cell fragmentation, Hydrogenase activity, Platymonas subcordiformis, Total protein concentration",

author = "Fei Yan and Zhao'an Chen and Hongbin Lu and Xingju Yu and Meifang Jin and Wei Zhang",

year = "2008",

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day = "1",

language = "English",

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pages = "497--500",

journal = "Hsi-An Chiao Tung Ta Hsueh/Journal of Xi'an Jiaotong University",

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TY - JOUR

T1 - Fragmentation of platymonas subcordiformis in anaerobic environment and effect on hydrogenase activity

AU - Yan, Fei

AU - Chen, Zhao'an

AU - Lu, Hongbin

AU - Yu, Xingju

AU - Jin, Meifang

AU - Zhang, Wei

PY - 2008/4/1

Y1 - 2008/4/1

N2 - To isolate and purify dissolvable hydrogenase in Platymonas subcordiformis cells, the scheme for alga cell fragmentation is designed in anaerobic environment, and the effects of experimental conditions on the hydrogenase activity are investigated. By means of ultrasonication, the concentration of total protein approaches to 2.73 g/L under the condition of 400 W output power, 5 s crushing time, 10 s interval for 20 times. The efficiency of hydrogenase isolation gets higher in case of glass beads or 0.2%(volume fraction) Triton X-100 to break up fresh cells to obtain 1.44 U/g and 1.27 U/g respectively, while the other proteins get less in crude extract by Triton X-100 method, which is suggested to gain enough purified hydrogenase.

AB - To isolate and purify dissolvable hydrogenase in Platymonas subcordiformis cells, the scheme for alga cell fragmentation is designed in anaerobic environment, and the effects of experimental conditions on the hydrogenase activity are investigated. By means of ultrasonication, the concentration of total protein approaches to 2.73 g/L under the condition of 400 W output power, 5 s crushing time, 10 s interval for 20 times. The efficiency of hydrogenase isolation gets higher in case of glass beads or 0.2%(volume fraction) Triton X-100 to break up fresh cells to obtain 1.44 U/g and 1.27 U/g respectively, while the other proteins get less in crude extract by Triton X-100 method, which is suggested to gain enough purified hydrogenase.

KW - Cell fragmentation

KW - Hydrogenase activity

KW - Platymonas subcordiformis

KW - Total protein concentration

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VL - 42

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JO - Hsi-An Chiao Tung Ta Hsueh/Journal of Xi'an Jiaotong University

JF - Hsi-An Chiao Tung Ta Hsueh/Journal of Xi'an Jiaotong University

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