Functional analysis of a type-I ribosome inactivating protein balsamin from Momordica balsaminawith anti-microbial and DNase activity.

Parminder Ajji, Ken Walder, Munish Puri

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg2+ (10–50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.

    Original languageEnglish
    Pages (from-to)265-271
    Number of pages7
    JournalPLANT FOODS FOR HUMAN NUTRITION
    Volume71
    Issue number3
    DOIs
    Publication statusPublished - 2016

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