TY - JOUR
T1 - Functional analysis of a type-I ribosome inactivating protein balsamin from Momordica balsaminawith anti-microbial and DNase activity.
AU - Ajji, Parminder
AU - Walder, Ken
AU - Puri, Munish
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg2+ (10–50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
AB - Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg2+ (10–50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
KW - Antibacterial
KW - Antioxidant
KW - Bioactive
KW - Nutraceutical
KW - Purification
KW - Therapeutic
UR - http://www.scopus.com/inward/record.url?scp=84975126621&partnerID=8YFLogxK
U2 - 10.1007/s11130-016-0555-4
DO - 10.1007/s11130-016-0555-4
M3 - Article
VL - 71
SP - 265
EP - 271
JO - PLANT FOODS FOR HUMAN NUTRITION
JF - PLANT FOODS FOR HUMAN NUTRITION
SN - 0921-9668
IS - 3
ER -