Fusion-protein-assisted protein crystallization

Bostjan Kobe, Thomas Ve, Simon Williams

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)


    Fusion proteins can be used directly in protein crystallization to assist crystallization in at least two different ways. In one approach, the 'heterologous fusion-protein approach', the fusion partner can provide additional surface area to promote crystal contact formation. In another approach, the 'fusion of interacting proteins approach', protein assemblies can be stabilized by covalently linking the interacting partners. The linker connecting the proteins plays different roles in the two applications: in the first approach a rigid linker is required to reduce conformational heterogeneity; in the second, conversely, a flexible linker is required that allows the native interaction between the fused proteins. The two approaches can also be combined. The recent applications of fusion-protein technology in protein crystallization from the work of our own and other laboratories are briefly reviewed.

    Original languageEnglish
    Pages (from-to)861-869
    Number of pages9
    JournalActa Crystallographica Section F-Structural Biology and Crystallization Communications
    Publication statusPublished - 1 Jan 2015


    • fusion of interacting proteins approach
    • heterologous fusion-protein approach
    • linker
    • membrane-protein crystallization
    • protein interactions
    • recombinant fusion protein


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