General Tissue Preparation Methods

John W. Stirling

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)
Original languageEnglish
Title of host publicationDiagnostic Electron Microscopy
Subtitle of host publicationA Practical Guide to Interpretation and Technique
EditorsJohn W Stirling, Alan Curry, Brian Eyden
PublisherJohn Wiley & Sons
Number of pages41
ISBN (Print)9781119973997
Publication statusPublished - 7 Jul 2013
Externally publishedYes

Bibliographical note

Copyright 2014 Elsevier B.V., All rights reserved.


  • Biomedical tissues, fixation and processing
  • Common sectioning problems and artefacts
  • Epoxy resins, embedding medium for standard biomedicals
  • Fixation and processing methods for diagnostic TEM
  • Fresh solid tissue from biopsies/fluids/suspensions
  • General tissue preparation methods
  • Reprocessing tissue, the 'Pop-Off' technique
  • Section contamination and staining artefacts
  • Tissue embedding and tissue sectioning
  • Tissue screening, rapid detection of diagnostic features

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