Generation and characterization of monoclonal antibodies to human type-5 tartrate-resistant acid phosphatase: Development of a specific immunoassay of the isoenzyme in serum

P. Chamberlain, J. Compston, T. M. Cox, A. R. Hayman, R. C. Imrie, K. Reynolds, S. D. Holmes

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Abstract

We have characterized four monoclonal antibodies (mAbs) to the purple ('tartrate-resistant,' band 5) acid phosphatase of the human osteoclast (TRAP) and used these to develop a specific serum immunoassay. All four mAbs are of high affinity (K(a) = 1-5 x 108 L/mol) with a very fast K(assoc) (0.2-2.0 x 105 L mol-1 s-1) and a moderate K(dissoc) (1-3 x 10-3 s). Two of the mAbs were selected to develop a time-resolved fluorescence immunoassay to measure serum concentrations of TRAP. The mean serum immunoreactive TRAP in a group of healthy premenopausal women and men was 3.7 ± 1.8 μg/L (mean ± SD) and 3.5 ± 1.6 μg/L, respectively. Significantly higher concentrations of TRAP were found in postmenopausal women (6.3 ± 2.3 μg/L) and in eight patients with Gaucher disease (19.3 ± 4.7 μg/L). Further studies are required to investigate the value of serum TRAP as a marker of bone resorption.

Original languageEnglish
Pages (from-to)1495-1499
Number of pages5
JournalClinical Chemistry
Volume41
Issue number10
DOIs
Publication statusPublished - 1 Oct 1995
Externally publishedYes

Keywords

  • bone disease
  • bone resorption

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