TY - JOUR
T1 - Generation of a New Tau Knockout (tau Δex1) Line Using CRISPR/Cas9 Genome Editing in Mice
AU - Tan, Daniel C.S.
AU - Yao, Sherilyn
AU - Ittner, Arne
AU - Bertz, Josefine
AU - Ke, Yazi D.
AU - Ittner, Lars M.
AU - Delerue, Fabien
PY - 2018
Y1 - 2018
N2 - Alzheimer's disease and other dementias present with tau pathology. Several mouse lines with knockout of the tau-encoding Mapt gene have been reported, yet findings often differed between lines and sites. Here, we report a new tau knockout strain (tau Δex1), generated by CRISPR/Cas9-mediated genome editing of intron -1/exon 1 of Mapt in C57Bl/6J mice. Tau Δex1 mice had no overt phenotype, but, in line with previous models, they showed a significantly reduced susceptibility to excitotoxic seizures, with normal memory formation in young mice. This new in vivo resource will be made freely available to the research community.
AB - Alzheimer's disease and other dementias present with tau pathology. Several mouse lines with knockout of the tau-encoding Mapt gene have been reported, yet findings often differed between lines and sites. Here, we report a new tau knockout strain (tau Δex1), generated by CRISPR/Cas9-mediated genome editing of intron -1/exon 1 of Mapt in C57Bl/6J mice. Tau Δex1 mice had no overt phenotype, but, in line with previous models, they showed a significantly reduced susceptibility to excitotoxic seizures, with normal memory formation in young mice. This new in vivo resource will be made freely available to the research community.
KW - C57Bl/6
KW - CRISPR
KW - gene knockout
KW - mouse
KW - tau
UR - http://www.scopus.com/inward/record.url?scp=85043597877&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/NHMRC/1037746
UR - http://purl.org/au-research/grants/NHMRC/1081916
UR - http://purl.org/au-research/grants/ARC/DP150104321
UR - http://purl.org/au-research/grants/ARC/DP170100781
UR - http://purl.org/au-research/grants/NHMRC/1123564
U2 - 10.3233/JAD-171058
DO - 10.3233/JAD-171058
M3 - Article
C2 - 29480201
AN - SCOPUS:85043597877
SN - 1387-2877
VL - 62
SP - 571
EP - 578
JO - Journal of Alzheimer's Disease
JF - Journal of Alzheimer's Disease
IS - 2
ER -