Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada; Kynon J.M. Benjamin; Anna C. Brandtjen; Ethan Tietze; Samuel J. Allen; Apuã C.M. Paquola; Joel E. Kleinman; Thomas M. Hyde; Jennifer A. Erwin

doi:10.1016/j.scr.2020.101806

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada, Kynon J.M. Benjamin, Anna C. Brandtjen, Ethan Tietze, Samuel J. Allen, Apuã C.M. Paquola, Joel E. Kleinman, Thomas M. Hyde, Jennifer A. Erwin

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Abstract

In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

Original language	English
Article number	101806
Number of pages	4
Journal	Stem Cell Research
Volume	46
DOIs	https://doi.org/10.1016/j.scr.2020.101806
Publication status	Published - Jul 2020
Externally published	Yes

Keywords

Induced pluripotent stem cell (iPSC)
transcriptomic
postmortem dura-derived fibroblasts
brain-region specific
low polygenic risk

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10.1016/j.scr.2020.101806Licence: CC BY

Published versionFinal published version, 1.36 MBLicence: CC BY

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Sawada, T., Benjamin, K. J. M., Brandtjen, A. C., Tietze, E., Allen, S. J., Paquola, A. C. M., Kleinman, J. E., Hyde, T. M., & Erwin, J. A. (2020). Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium. Stem Cell Research, 46, Article 101806. https://doi.org/10.1016/j.scr.2020.101806

@article{30fcc91c08344bd298206ac74ec24674,

title = "Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.",

abstract = "In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.",

keywords = "Induced pluripotent stem cell (iPSC), transcriptomic, postmortem dura-derived fibroblasts, brain-region specific, low polygenic risk",

author = "Tomoyo Sawada and Benjamin, {Kynon J.M.} and Brandtjen, {Anna C.} and Ethan Tietze and Allen, {Samuel J.} and Paquola, {Apu{\~a} C.M.} and Kleinman, {Joel E.} and Hyde, {Thomas M.} and Erwin, {Jennifer A.}",

year = "2020",

month = jul,

doi = "10.1016/j.scr.2020.101806",

language = "English",

volume = "46",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

Sawada, T, Benjamin, KJM, Brandtjen, AC, Tietze, E, Allen, SJ, Paquola, ACM, Kleinman, JE, Hyde, TM & Erwin, JA 2020, 'Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.', Stem Cell Research, vol. 46, 101806. https://doi.org/10.1016/j.scr.2020.101806

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium. / Sawada, Tomoyo; Benjamin, Kynon J.M.; Brandtjen, Anna C. et al.
In: Stem Cell Research, Vol. 46, 101806, 07.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

AU - Sawada, Tomoyo

AU - Benjamin, Kynon J.M.

AU - Brandtjen, Anna C.

AU - Tietze, Ethan

AU - Allen, Samuel J.

AU - Paquola, Apuã C.M.

AU - Kleinman, Joel E.

AU - Hyde, Thomas M.

AU - Erwin, Jennifer A.

PY - 2020/7

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N2 - In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

AB - In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

KW - Induced pluripotent stem cell (iPSC)

KW - transcriptomic

KW - postmortem dura-derived fibroblasts

KW - brain-region specific

KW - low polygenic risk

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DO - 10.1016/j.scr.2020.101806

M3 - Article

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AN - SCOPUS:85084823978

SN - 1873-5061

VL - 46

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M1 - 101806

ER -

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

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Keywords

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