TY - JOUR
T1 - Generation of reactive oxygen species from porous silicon microparticles in cell culture medium
AU - Low, Suet
AU - Williams, Keryn
AU - Canham, Leigh
AU - Voelcker, Nicolas
PY - 2010/6/1
Y1 - 2010/6/1
N2 - Nanostructured (porous) silicon is a promising biodegradable biomaterial, which is being intensively researched as a tissue engineering scaffold and drugdelivery vehicle. Here, we tested the biocompatibility of non-treated and thermally-oxidized porous silicon particles using an indirect cell viability assay. Initial direct cell culture on porous silicon determined that human lens epithelial cells only poorly adhered to non-treated porous silicon. Using an indirect cell culture assay, we found that non-treated microparticles caused complete cell death, indicating that these particles generated a toxic product in cell culture medium. In contrast, thermally-oxidized microparticles did not reduce cell viability significantly. We found evidence for the generation of reactive oxygen species (ROS) by means of the fluorescent probe 2′,7′-dichlorofluorescin. Our results suggest that non-treated porous silicon microparticles produced ROS, which interacted with the components of the cell culture medium, leading to the formation of cytotoxic species. Oxidation of porous silicon microparticles not only mitigated, but also abolished the toxic effects.
AB - Nanostructured (porous) silicon is a promising biodegradable biomaterial, which is being intensively researched as a tissue engineering scaffold and drugdelivery vehicle. Here, we tested the biocompatibility of non-treated and thermally-oxidized porous silicon particles using an indirect cell viability assay. Initial direct cell culture on porous silicon determined that human lens epithelial cells only poorly adhered to non-treated porous silicon. Using an indirect cell culture assay, we found that non-treated microparticles caused complete cell death, indicating that these particles generated a toxic product in cell culture medium. In contrast, thermally-oxidized microparticles did not reduce cell viability significantly. We found evidence for the generation of reactive oxygen species (ROS) by means of the fluorescent probe 2′,7′-dichlorofluorescin. Our results suggest that non-treated porous silicon microparticles produced ROS, which interacted with the components of the cell culture medium, leading to the formation of cytotoxic species. Oxidation of porous silicon microparticles not only mitigated, but also abolished the toxic effects.
KW - Biodegradable materials
KW - Microparticles
KW - Porous silicon
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=77950676476&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.32610
DO - 10.1002/jbm.a.32610
M3 - Article
VL - 93
SP - 1124
EP - 1131
JO - Journal of Biomedical Materials Research Part A
JF - Journal of Biomedical Materials Research Part A
SN - 1549-3296
IS - 3
ER -