Genetic speciation of Candida isolates by arbitrarily primed polymerase chain reaction

D. Liu, S. Coloe, S. Lloyd Jones, R. Baird, J. Pedersen

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11 Citations (Scopus)


Candida species are opportunistic human pathogens capable of causing a variety of clinical diseases. Rapid and precise identification of Candida to species level is essential for effective treatment and management strategies. Conventional diagnosis of candidiasis is sometimes slow and variable. By using a random primer 5'-ACGGGCCAGT-3' in arbitrarily primed polymerase chain reaction, seven common Candida species (i.e., C. albicans, C. guilliermondii, C. parapsilosis, C, krusei, C. lipolytica, C. tropicalis C. (Torulopsis) glabrata) produced characteristic DNA band patterns, which enabled rapid determination to species level among them. Further analysis of the nucleotide sequences of Candida specific fragments should improve our understanding of the molecular structures and possible functions of the gene regions involved.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - Nov 1996
Externally publishedYes


  • Arbitrarily primed polymerase chain reaction
  • Candida
  • Fungus
  • Speciation
  • Yeast


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