Genome complexity of the maize rust fungus,Puccinia sorghi

P. A. Anderson; B. M. Tyler; A. Pryor

doi:10.1016/0147-5975(92)90006-D

Genome complexity of the maize rust fungus,Puccinia sorghi

P. A. Anderson, B. M. Tyler, A. Pryor

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7 Citations (Scopus)

Abstract

The base composition and complexity of genomic DNA fromPuccinia sorghi have been estimated by thermal denaturation, analytical ultracentrifugation, and reassociation kinetics. The buoyant density of genomic DNA in CsCl was found to be 1.7021 g/ml, which corresponds to a GC content of 43%. From thermal denaturation curves the GC content was estimated to be 41%. The haploid genome size ofP. sorghi was estimated to be 4.7 × 10⁷ bp, half of which represented a moderately repetitive fraction. The size of theP. sorghi genome is similar to that of other basidiomycete fungi; however, the amount of repetitive DNA is greater than that reported for most other fungi.

Original language	English
Pages (from-to)	302-307
Number of pages	6
Journal	Experimental Mycology
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/0147-5975(92)90006-D
Publication status	Published - Dec 1992
Externally published	Yes

Keywords

Puccinia sorghi
rust fungi
Zea mays

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title = "Genome complexity of the maize rust fungus,Puccinia sorghi",

abstract = "The base composition and complexity of genomic DNA fromPuccinia sorghi have been estimated by thermal denaturation, analytical ultracentrifugation, and reassociation kinetics. The buoyant density of genomic DNA in CsCl was found to be 1.7021 g/ml, which corresponds to a GC content of 43%. From thermal denaturation curves the GC content was estimated to be 41%. The haploid genome size ofP. sorghi was estimated to be 4.7 × 107 bp, half of which represented a moderately repetitive fraction. The size of theP. sorghi genome is similar to that of other basidiomycete fungi; however, the amount of repetitive DNA is greater than that reported for most other fungi.",

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author = "Anderson, {P. A.} and Tyler, {B. M.} and A. Pryor",

year = "1992",

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T1 - Genome complexity of the maize rust fungus,Puccinia sorghi

AU - Anderson, P. A.

AU - Tyler, B. M.

AU - Pryor, A.

PY - 1992/12

Y1 - 1992/12

N2 - The base composition and complexity of genomic DNA fromPuccinia sorghi have been estimated by thermal denaturation, analytical ultracentrifugation, and reassociation kinetics. The buoyant density of genomic DNA in CsCl was found to be 1.7021 g/ml, which corresponds to a GC content of 43%. From thermal denaturation curves the GC content was estimated to be 41%. The haploid genome size ofP. sorghi was estimated to be 4.7 × 107 bp, half of which represented a moderately repetitive fraction. The size of theP. sorghi genome is similar to that of other basidiomycete fungi; however, the amount of repetitive DNA is greater than that reported for most other fungi.

AB - The base composition and complexity of genomic DNA fromPuccinia sorghi have been estimated by thermal denaturation, analytical ultracentrifugation, and reassociation kinetics. The buoyant density of genomic DNA in CsCl was found to be 1.7021 g/ml, which corresponds to a GC content of 43%. From thermal denaturation curves the GC content was estimated to be 41%. The haploid genome size ofP. sorghi was estimated to be 4.7 × 107 bp, half of which represented a moderately repetitive fraction. The size of theP. sorghi genome is similar to that of other basidiomycete fungi; however, the amount of repetitive DNA is greater than that reported for most other fungi.

KW - Puccinia sorghi

KW - rust fungi

KW - Zea mays

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DO - 10.1016/0147-5975(92)90006-D

M3 - Article

AN - SCOPUS:38249008084

SN - 0147-5975

VL - 16

SP - 302

EP - 307

JO - Experimental Mycology

JF - Experimental Mycology

IS - 4

ER -

Genome complexity of the maize rust fungus,Puccinia sorghi

Abstract

Keywords

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