Genome complexity of the maize rust fungus,Puccinia sorghi

P. A. Anderson, B. M. Tyler, A. Pryor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The base composition and complexity of genomic DNA fromPuccinia sorghi have been estimated by thermal denaturation, analytical ultracentrifugation, and reassociation kinetics. The buoyant density of genomic DNA in CsCl was found to be 1.7021 g/ml, which corresponds to a GC content of 43%. From thermal denaturation curves the GC content was estimated to be 41%. The haploid genome size ofP. sorghi was estimated to be 4.7 × 107 bp, half of which represented a moderately repetitive fraction. The size of theP. sorghi genome is similar to that of other basidiomycete fungi; however, the amount of repetitive DNA is greater than that reported for most other fungi.

Original languageEnglish
Pages (from-to)302-307
Number of pages6
JournalExperimental Mycology
Volume16
Issue number4
DOIs
Publication statusPublished - Dec 1992
Externally publishedYes

Keywords

  • Puccinia sorghi
  • rust fungi
  • Zea mays

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