TY - JOUR
T1 - Genome-wide study of the defective sucrose fermenter strain of vibrio cholerae from the Latin American cholera epidemic
AU - Garza, Daniel Rios
AU - Thompson, Cristiane C.
AU - Loureiro, Edvaldo Carlos Brito
AU - Dutilh, Bas E.
AU - Inada, Davi Toshio
AU - Junior, Edivaldo Costa Sousa
AU - Cardoso, Jedson Ferreira
AU - Nunes, Márcio Roberto T.
AU - de Lima, Clayton Pereira Silva
AU - Silvestre, Rodrigo Vellasco Duarte
AU - Nunes, Keley Nascimento Barbosa
AU - Santos, Elisabeth C.O.
AU - Edwards, Robert A.
AU - Vicente, Ana Carolina P.
AU - de Sá Morais, Lena Lillian Canto
N1 - This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2012/5/25
Y1 - 2012/5/25
N2 - The 7th cholera pandemic reached Latin America in 1991, spreading from Peru to virtually all Latin American countries. During the late epidemic period, a strain that failed to ferment sucrose dominated cholera outbreaks in the Northern Brazilian Amazon region. In order to understand the genomic characteristics and the determinants of this altered sucrose fermenting phenotype, the genome of the strain IEC224 was sequenced. This paper reports a broad genomic study of this strain, showing its correlation with the major epidemic lineage. The potentially mobile genomic regions are shown to possess GC content deviation, and harbor the main V. cholera virulence genes. A novel bioinformatic approach was applied in order to identify the putative functions of hypothetical proteins, and was compared with the automatic annotation by RAST. The genome of a large bacteriophage was found to be integrated to the IEC224's alanine aminopeptidase gene. The presence of this phage is shown to be a common characteristic of the El Tor strains from the Latin American epidemic, as well as its putative ancestor from Angola. The defective sucrose fermenting phenotype is shown to be due to a single nucleotide insertion in the V. cholerae sucrose-specific transportation gene. This frame-shift mutation truncated a membrane protein, altering its structural pore-like conformation. Further, the identification of a common bacteriophage reinforces both the monophyletic and African-Origin hypotheses for the main causative agent of the 1991 Latin America cholera epidemics.
AB - The 7th cholera pandemic reached Latin America in 1991, spreading from Peru to virtually all Latin American countries. During the late epidemic period, a strain that failed to ferment sucrose dominated cholera outbreaks in the Northern Brazilian Amazon region. In order to understand the genomic characteristics and the determinants of this altered sucrose fermenting phenotype, the genome of the strain IEC224 was sequenced. This paper reports a broad genomic study of this strain, showing its correlation with the major epidemic lineage. The potentially mobile genomic regions are shown to possess GC content deviation, and harbor the main V. cholera virulence genes. A novel bioinformatic approach was applied in order to identify the putative functions of hypothetical proteins, and was compared with the automatic annotation by RAST. The genome of a large bacteriophage was found to be integrated to the IEC224's alanine aminopeptidase gene. The presence of this phage is shown to be a common characteristic of the El Tor strains from the Latin American epidemic, as well as its putative ancestor from Angola. The defective sucrose fermenting phenotype is shown to be due to a single nucleotide insertion in the V. cholerae sucrose-specific transportation gene. This frame-shift mutation truncated a membrane protein, altering its structural pore-like conformation. Further, the identification of a common bacteriophage reinforces both the monophyletic and African-Origin hypotheses for the main causative agent of the 1991 Latin America cholera epidemics.
UR - http://www.scopus.com/inward/record.url?scp=84861475624&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0037283
DO - 10.1371/journal.pone.0037283
M3 - Article
AN - SCOPUS:84861475624
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 5
M1 - e37283
ER -