GENOMIC MHC HAPLOTYPING OF MUTANT CELL LINES USING A NOVEL MARKER

M. A. Degli‐Esposti, J. F. Williamson, G. K. Tay, B. Salmon, G. A. Joseph, A. A. Morley, R. L. Dawkins

Research output: Contribution to journalArticle

Abstract

The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti‐HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region of the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HLA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalInternational Journal of Immunogenetics
Volume20
Issue number5
DOIs
Publication statusPublished - Oct 1993
Externally publishedYes

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    Degli‐Esposti, M. A., Williamson, J. F., Tay, G. K., Salmon, B., Joseph, G. A., Morley, A. A., & Dawkins, R. L. (1993). GENOMIC MHC HAPLOTYPING OF MUTANT CELL LINES USING A NOVEL MARKER. International Journal of Immunogenetics, 20(5), 373-380. https://doi.org/10.1111/j.1744-313X.1993.tb00156.x