Growth and lactic acid production in batch culture of Lactobacillus rhamnosus in a defined medium

Andrew R. Berry, Christopher M.M. Franco, Wei Zhang, Anton P.J. Middelberg

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    61 Citations (Scopus)


    To facilitate metabolic analysis, batch fermentations of Lactobacillus rhamnosus were carried out in a new defined medium. Biomass at 10.5 g/l and lactic acid at 67 g/l with a Y(P/S) of 0.84 were achieved. The maximum specific growth rate and the average productivity were 0.49/h and 2.48 g/l.h, respectively. These are comparable to those of this organism and related organisms in complex media. Preliminary amino acid studies were also conducted, highlighting the importance of serine, asparagine, glutamine and cysteine. Kinetic analysis revealed that lactic acid production was predominantly growth-associated with growth associated and non-growth associated lactic acid constants of 0.389 mol/g-cell and 0.0025 mol/g-cell.h, respectively. Finally a kinetic model has been included to describe the fermentation of L. rhamnosus.

    Original languageEnglish
    Pages (from-to)163-167
    Number of pages5
    JournalBiotechnology Letters
    Issue number2
    Publication statusPublished - 1 Feb 1999


    • Defined medium
    • Kinetic modeling
    • Lactic acid
    • Lactobacillus rhamnosus


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