Growth and the environment of Schizosaccharomyces pombe

Janni Petersen, Paul Russell

    Research output: Contribution to journalArticlepeer-review

    54 Citations (Scopus)

    Abstract

    Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures. We discuss the concept of “culture memory,” in which the growth state and stress experienced by a strain during storage, propagation, and starter culture preparation can alter experimental outcomes at later stages. We also describe the triggers that are widely used to manipulate signaling through the environment sensing pathways.

    Original languageEnglish
    Pages (from-to)210-226
    Number of pages17
    JournalCold Spring Harbor Protocols
    Volume2016
    Issue number3
    DOIs
    Publication statusPublished - Mar 2016

    Keywords

    • Cell Biology
    • Cell Culture
    • Yeast Genetics
    • Laboratory Organisms
    • Yeast

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