Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma

Bianca Sampaio Dotto Fiuza; Candace Machado de Andrade; Pedro Milet Meirelles; Jorley Santos da Silva; Milca de Jesus Silva; Cinthia Vila Nova Santana; Gabriela Pimentel Pinheiro; Harriet Mpairwe; Philip Cooper; Collin Brooks; Lucy Pembrey; Steven Taylor; Jeroen Douwes; Álvaro A. Cruz; Mauricio L. Barreto; Neil Pearce; Camila A.V. Figueiredo

doi:10.1016/j.jacig.2024.100242

Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma

Bianca Sampaio Dotto Fiuza, Candace Machado de Andrade, Pedro Milet Meirelles, Jorley Santos da Silva, Milca de Jesus Silva, Cinthia Vila Nova Santana, Gabriela Pimentel Pinheiro, Harriet Mpairwe, Philip Cooper, Collin Brooks, Lucy Pembrey, Steven Taylor, Jeroen Douwes, Álvaro A. Cruz, Mauricio L. Barreto, Neil Pearce, Camila A.V. Figueiredo

College of Medicine and Public Health

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

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Abstract

Background: Asthma is a complex disease and a severe global public health problem resulting from interactions between genetic background and environmental exposures. It has been suggested that gut microbiota may be related to asthma development; however, such relationships needs further investigation.

Objective: This study aimed to characterize the gut microbiota as well as the nasal lavage cytokine profile of asthmatic and nonasthmatic individuals.

Methods: Stool and nasal lavage samples were collected from 29 children and adolescents with type 2 asthma and 28 children without asthma in Brazil. Amplicon sequencing of the stool bacterial V4 region of the 16S rRNA gene was performed using Illumina MiSeq. Microbiota analysis was performed by QIIME 2 and PICRUSt2. Type 2 asthma phenotype was characterized by high sputum eosinophil counts and positive skin prick tests for house dust mite, cockroach, and/or cat or dog dander. The nasal immune marker profile was assessed using a customized multiplex panel.

Results: Stool microbiota differed significantly between asthmatic and nonasthmatic participants (P = .001). Bacteroides was more abundant in participants with asthma (P < .05), while Prevotella was more abundant in nonasthmatic individuals (P < .05). In people with asthma, the relative abundance of Bacteroides correlated with IL-4 concentration in nasal lavage samples. Inference of microbiota functional capacity identified differential fatty acid biosynthesis in asthmatic compared to nonasthmatic subjects.

Conclusion: The stool microbiota differed between asthmatic and nonasthmatic young people in Brazil. Asthma was associated with higher Bacteroides levels, which correlated with nasal IL-4 concentration.

Original language	English
Article number	100242
Number of pages	10
Journal	Journal of Allergy and Clinical Immunology: Global
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.jacig.2024.100242
Publication status	Published - May 2024

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Asthma
gut microbiota
microbiome
stool

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10.1016/j.jacig.2024.100242Licence: CC BY-NC-ND

Final published versionFinal published version, 1.97 MBLicence: CC BY-NC-ND

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Sampaio Dotto Fiuza, B., Machado de Andrade, C., Meirelles, P. M., Santos da Silva, J., de Jesus Silva, M., Vila Nova Santana, C., Pimentel Pinheiro, G., Mpairwe, H., Cooper, P., Brooks, C., Pembrey, L., Taylor, S., Douwes, J., Cruz, Á. A., Barreto, M. L., Pearce, N., & Figueiredo, C. A. V. (2024). Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma. Journal of Allergy and Clinical Immunology: Global, 3(2), Article 100242. https://doi.org/10.1016/j.jacig.2024.100242

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title = "Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma",

abstract = "Background: Asthma is a complex disease and a severe global public health problem resulting from interactions between genetic background and environmental exposures. It has been suggested that gut microbiota may be related to asthma development; however, such relationships needs further investigation. Objective: This study aimed to characterize the gut microbiota as well as the nasal lavage cytokine profile of asthmatic and nonasthmatic individuals. Methods: Stool and nasal lavage samples were collected from 29 children and adolescents with type 2 asthma and 28 children without asthma in Brazil. Amplicon sequencing of the stool bacterial V4 region of the 16S rRNA gene was performed using Illumina MiSeq. Microbiota analysis was performed by QIIME 2 and PICRUSt2. Type 2 asthma phenotype was characterized by high sputum eosinophil counts and positive skin prick tests for house dust mite, cockroach, and/or cat or dog dander. The nasal immune marker profile was assessed using a customized multiplex panel. Results: Stool microbiota differed significantly between asthmatic and nonasthmatic participants (P = .001). Bacteroides was more abundant in participants with asthma (P < .05), while Prevotella was more abundant in nonasthmatic individuals (P < .05). In people with asthma, the relative abundance of Bacteroides correlated with IL-4 concentration in nasal lavage samples. Inference of microbiota functional capacity identified differential fatty acid biosynthesis in asthmatic compared to nonasthmatic subjects. Conclusion: The stool microbiota differed between asthmatic and nonasthmatic young people in Brazil. Asthma was associated with higher Bacteroides levels, which correlated with nasal IL-4 concentration.",

keywords = "Asthma, gut microbiota, microbiome, stool",

author = "{Sampaio Dotto Fiuza}, Bianca and {Machado de Andrade}, Candace and Meirelles, {Pedro Milet} and {Santos da Silva}, Jorley and {de Jesus Silva}, Milca and {Vila Nova Santana}, Cinthia and {Pimentel Pinheiro}, Gabriela and Harriet Mpairwe and Philip Cooper and Collin Brooks and Lucy Pembrey and Steven Taylor and Jeroen Douwes and Cruz, {{\'A}lvaro A.} and Barreto, {Mauricio L.} and Neil Pearce and Figueiredo, {Camila A.V.}",

year = "2024",

month = may,

doi = "10.1016/j.jacig.2024.100242",

language = "English",

volume = "3",

journal = "Journal of Allergy and Clinical Immunology: Global",

issn = "2772-8293",

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Sampaio Dotto Fiuza, B, Machado de Andrade, C, Meirelles, PM, Santos da Silva, J, de Jesus Silva, M, Vila Nova Santana, C, Pimentel Pinheiro, G, Mpairwe, H, Cooper, P, Brooks, C, Pembrey, L, Taylor, S, Douwes, J, Cruz, ÁA, Barreto, ML, Pearce, N & Figueiredo, CAV 2024, 'Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma', Journal of Allergy and Clinical Immunology: Global, vol. 3, no. 2, 100242. https://doi.org/10.1016/j.jacig.2024.100242

TY - JOUR

T1 - Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma

AU - Sampaio Dotto Fiuza, Bianca

AU - Machado de Andrade, Candace

AU - Meirelles, Pedro Milet

AU - Santos da Silva, Jorley

AU - de Jesus Silva, Milca

AU - Vila Nova Santana, Cinthia

AU - Pimentel Pinheiro, Gabriela

AU - Mpairwe, Harriet

AU - Cooper, Philip

AU - Brooks, Collin

AU - Pembrey, Lucy

AU - Taylor, Steven

AU - Douwes, Jeroen

AU - Cruz, Álvaro A.

AU - Barreto, Mauricio L.

AU - Pearce, Neil

AU - Figueiredo, Camila A.V.

PY - 2024/5

Y1 - 2024/5

N2 - Background: Asthma is a complex disease and a severe global public health problem resulting from interactions between genetic background and environmental exposures. It has been suggested that gut microbiota may be related to asthma development; however, such relationships needs further investigation. Objective: This study aimed to characterize the gut microbiota as well as the nasal lavage cytokine profile of asthmatic and nonasthmatic individuals. Methods: Stool and nasal lavage samples were collected from 29 children and adolescents with type 2 asthma and 28 children without asthma in Brazil. Amplicon sequencing of the stool bacterial V4 region of the 16S rRNA gene was performed using Illumina MiSeq. Microbiota analysis was performed by QIIME 2 and PICRUSt2. Type 2 asthma phenotype was characterized by high sputum eosinophil counts and positive skin prick tests for house dust mite, cockroach, and/or cat or dog dander. The nasal immune marker profile was assessed using a customized multiplex panel. Results: Stool microbiota differed significantly between asthmatic and nonasthmatic participants (P = .001). Bacteroides was more abundant in participants with asthma (P < .05), while Prevotella was more abundant in nonasthmatic individuals (P < .05). In people with asthma, the relative abundance of Bacteroides correlated with IL-4 concentration in nasal lavage samples. Inference of microbiota functional capacity identified differential fatty acid biosynthesis in asthmatic compared to nonasthmatic subjects. Conclusion: The stool microbiota differed between asthmatic and nonasthmatic young people in Brazil. Asthma was associated with higher Bacteroides levels, which correlated with nasal IL-4 concentration.

AB - Background: Asthma is a complex disease and a severe global public health problem resulting from interactions between genetic background and environmental exposures. It has been suggested that gut microbiota may be related to asthma development; however, such relationships needs further investigation. Objective: This study aimed to characterize the gut microbiota as well as the nasal lavage cytokine profile of asthmatic and nonasthmatic individuals. Methods: Stool and nasal lavage samples were collected from 29 children and adolescents with type 2 asthma and 28 children without asthma in Brazil. Amplicon sequencing of the stool bacterial V4 region of the 16S rRNA gene was performed using Illumina MiSeq. Microbiota analysis was performed by QIIME 2 and PICRUSt2. Type 2 asthma phenotype was characterized by high sputum eosinophil counts and positive skin prick tests for house dust mite, cockroach, and/or cat or dog dander. The nasal immune marker profile was assessed using a customized multiplex panel. Results: Stool microbiota differed significantly between asthmatic and nonasthmatic participants (P = .001). Bacteroides was more abundant in participants with asthma (P < .05), while Prevotella was more abundant in nonasthmatic individuals (P < .05). In people with asthma, the relative abundance of Bacteroides correlated with IL-4 concentration in nasal lavage samples. Inference of microbiota functional capacity identified differential fatty acid biosynthesis in asthmatic compared to nonasthmatic subjects. Conclusion: The stool microbiota differed between asthmatic and nonasthmatic young people in Brazil. Asthma was associated with higher Bacteroides levels, which correlated with nasal IL-4 concentration.

KW - Asthma

KW - gut microbiota

KW - microbiome

KW - stool

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U2 - 10.1016/j.jacig.2024.100242

DO - 10.1016/j.jacig.2024.100242

M3 - Article

AN - SCOPUS:85189084414

SN - 2772-8293

VL - 3

JO - Journal of Allergy and Clinical Immunology: Global

JF - Journal of Allergy and Clinical Immunology: Global

IS - 2

M1 - 100242

ER -

Gut microbiome signature and nasal lavage inflammatory markers in young people with asthma

Abstract

UN SDGs

Keywords

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