Gut microbiota composition does not associate with Toxoplasma infection in rats

Patrick L. Taggart, Craig Liddicoat, Wen Han Tong, Martin F. Breed, Philip Weinstein, David Wheeler, Ajai Vyas

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
72 Downloads (Pure)

Abstract

Toxoplasma infection in intermediate host species closely associates with inflammation. This association has led to suggestions that the behavioural changes associated with infection may be indirectly driven by the resulting sustained inflammation rather than a direct behavioural manipulation by the parasite. If this is correct, sustained inflammation in chronically infected rodents should present as widespread differences in the gastrointestinal microbiota due to the dependency between the composition of these microbiota and sustained inflammation. We conducted a randomized controlled experiment in rats that were assigned to a Toxoplasma-treatment, placebo-treatment or negative control group. We euthanised rats during the chronic phase of infection, collected their caecal stool samples and sequenced the V3-V4 region of the 16S rRNA gene to characterize the bacterial community in these samples. Toxoplasma infection did not induce widespread differences in the bacterial community composition of the gastrointestinal tract of rats. Rather, we found sex differences in the bacterial community composition of rats. We conclude that it is unlikely that sustained inflammation is the mechanism driving the highly specific behavioural changes observed in Toxoplasma-positive rats.

Original languageEnglish
Pages (from-to)3963-3970
Number of pages8
JournalMolecular Ecology
Volume31
Issue number14
Early online date27 May 2022
DOIs
Publication statusPublished - Jul 2022

Keywords

  • Toxoplasma
  • microbiome
  • microbiota
  • inflammation
  • parasite
  • toxoplasma
  • behavioural manipulation

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