TY - JOUR
T1 - Haemopoietic progenitor cells measured by limiting dilution in microwell culture.
AU - Wan, J. H.
AU - Morley, A. A.
PY - 1985/10
Y1 - 1985/10
N2 - Microwell culture assays, termed the "negative" well technique and the "positive" well technique have been used to measure the concentration of murine haemopoietic progenitor cells (HPC) by limiting dilution. Using morphologic examination and 3H-TdR uptake, the number of HPC of Balb/c mice per 10(5) bone marrow cells was 33 and 31 respectively. the linear relation between the number of harvested cells and input cells seen on the 4th and 7th days of culture reflected the growth of HPC in microwells. Besides measuring the concentration of HPC, this culture system may facilitate the analysis of effects on proliferation and differentiation of HPC of culture conditions, haemopoietic factors as well as cytotoxic agents.
AB - Microwell culture assays, termed the "negative" well technique and the "positive" well technique have been used to measure the concentration of murine haemopoietic progenitor cells (HPC) by limiting dilution. Using morphologic examination and 3H-TdR uptake, the number of HPC of Balb/c mice per 10(5) bone marrow cells was 33 and 31 respectively. the linear relation between the number of harvested cells and input cells seen on the 4th and 7th days of culture reflected the growth of HPC in microwells. Besides measuring the concentration of HPC, this culture system may facilitate the analysis of effects on proliferation and differentiation of HPC of culture conditions, haemopoietic factors as well as cytotoxic agents.
UR - http://www.scopus.com/inward/record.url?scp=0022132373&partnerID=8YFLogxK
UR - https://europepmc.org/article/med/3837322
M3 - Article
C2 - 3837322
AN - SCOPUS:0022132373
SN - 0253-5823
VL - 28
SP - 1070
EP - 1078
JO - Scientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences / Chung-kuo k"o hsueh yuan, chu pan
JF - Scientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences / Chung-kuo k"o hsueh yuan, chu pan
IS - 10
ER -