HCV RNA traffic and association with NS5A in living cells

Guillaume Fiches, Nicholas Eyre, Amanda Aloia, Kylie Van Der Hoek, Brigit Betz-Stablein, Fabio Luciani, Abha Chopra, Michael Beard

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The spatiotemporal dynamics of Hepatitis C Virus (HCV) RNA localisation are poorly understood. To address this we engineered HCV genomes harbouring MS2 bacteriophage RNA stem-loops within the 3'-untranslated region to allow tracking of HCV RNA via specific interaction with a MS2-Coat-mCherry fusion protein. Despite the impact of these insertions on viral fitness, live imaging revealed that replication of tagged-HCV genomes induced specific redistribution of the mCherry-tagged-MS2-Coat protein to motile and static foci. Further analysis showed that HCV RNA was associated with NS5A in both static and motile structures while a subset of motile NS5A structures was devoid of HCV RNA. Further investigation of viral RNA traffic with respect to lipid droplets (LDs) revealed HCV RNA-positive structures in close association with LDs. These studies provide new insights into the dynamics of HCV RNA traffic with NS5A and LDs and provide a platform for future investigations of HCV replication and assembly.

    Original languageEnglish
    Pages (from-to)60-74
    Number of pages15
    JournalVirology
    Volume493
    DOIs
    Publication statusPublished - 2016

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  • Cite this

    Fiches, G., Eyre, N., Aloia, A., Van Der Hoek, K., Betz-Stablein, B., Luciani, F., Chopra, A., & Beard, M. (2016). HCV RNA traffic and association with NS5A in living cells. Virology, 493, 60-74. https://doi.org/10.1016/j.virol.2016.02.016