HDCR: A novel method for the diagnostic detection of methylated DNA for blood based cancer diagnostics

K. Rand, P. Molloy, T. Ho, G. P. Young

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    Release of hypermethylated DNA from tumour cells affords the opportunity to develop an early diagnostic for cancer by DNA isolated from blood or other bodily fluids. However, using standard methylation-specific PCR, DNA frist needs to be treated with bisulfite and sensitivity can be limited due to mispriming. Once formed, unwanted products amplify efficiently and can prevent detection of the target. To overcome this we have developed a system which does not require bisulfite treatment of DNA and in which selectivity is maintained throughout the amplification.

    Original languageEnglish
    Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
    Pages81-84
    Number of pages4
    Publication statusPublished - 12 May 2013
    EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
    Duration: 12 May 201316 May 2013

    Publication series

    NameTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
    Volume3

    Conference

    ConferenceNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
    CountryUnited States
    CityWashington, DC
    Period12/05/1316/05/13

    Keywords

    • Blood-based
    • Cancer
    • Diagnostic
    • PCR

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