Abstract
Release of hypermethylated DNA from tumour cells affords the opportunity to develop an early diagnostic for cancer by DNA isolated from blood or other bodily fluids. However, using standard methylation-specific PCR, DNA frist needs to be treated with bisulfite and sensitivity can be limited due to mispriming. Once formed, unwanted products amplify efficiently and can prevent detection of the target. To overcome this we have developed a system which does not require bisulfite treatment of DNA and in which selectivity is maintained throughout the amplification.
| Original language | English |
|---|---|
| Title of host publication | Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 |
| Pages | 81-84 |
| Number of pages | 4 |
| Publication status | Published - 12 May 2013 |
| Event | Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States Duration: 12 May 2013 → 16 May 2013 |
Publication series
| Name | Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 |
|---|---|
| Volume | 3 |
Conference
| Conference | Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 |
|---|---|
| Country/Territory | United States |
| City | Washington, DC |
| Period | 12/05/13 → 16/05/13 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Blood-based
- Cancer
- Diagnostic
- PCR
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