Herceptin functionalized microfluidic polydimethylsiloxane devices for the capture of human epidermal growth factor receptor 2 positive circulating breast cancer cells

Benjamin Thierry, Mahaveer Kurkuri, Jun Yan Shi, Lwin Ei Mon Phyo Lwin, Dennis Palms

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Building on recent breakthroughs in the field of microfluidic-based capture of rare cancer cells circulating in the blood, the present article reports on the use of Herceptin functionalized PDMS devices designed to efficiently capture from blood cancer cells, overexpressing the tyrosine kinase human epidermal growth factor receptor (HER2). The identification of patients overexpressing HER2 is critical as it typically associates with an aggressive disease course in breast cancer and poor prognosis. Importantly, HER2 positive patients have been found to significantly benefit from Herceptin (Trastuzumab), a humanized monoclonal antibody (MAb) against HER2. Disposable PDMS devices prepared using standard soft lithography were functionalized by the plasma polymerization of an epoxy-containing monomer. The epoxy-rich thin film (AGEpp) thus created could be conjugated with Herceptin either directly or through a polyethylene glycol interlayer. The properties and reactivity toward the monoclonal antibody conjugation of these coatings were determined using x-ray photoelectron spectroscopy; direct conjugation provided a good compromise in reactivity and resistance to biologically nonspecific fouling and was selected. Using the breast cancer cell line SK-BR-3 as a model for cells overexpressing HER2, the immunocapture efficacy of the Herceptin functionalized PDMS was demonstrated in model studies. Validation studies confirmed the ability of the device to efficiently capture (~80% capture yield) HER2 positive cells from full blood.

Original languageEnglish
Article number032205
Number of pages10
JournalBiomicrofluidics
Volume4
Issue number3
DOIs
Publication statusPublished - Sep 2010
Externally publishedYes

Keywords

  • breast cancer cells
  • human epidermal growth
  • Herceptin
  • PDMS
  • blood cancer cells

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