High-resolution analysis of cis-acting regulatory networks at the α-globin locus

Jim Hughes, Karen Lower, Ian Dunham, Stephen Taylor, Marco De Gobbi, Jackie Sloane-Stanley, Simon McGowan, Jiannis Ragoussis, Douglas Vernimmen, Richard Gibbons, Doug Higgs

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    9 Citations (Scopus)

    Abstract

    We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the a-globin cluster.

    Original languageEnglish
    Pages (from-to)20120361
    Number of pages11
    JournalPhilosophical Transactions of the Royal Society of London Series B: Biological Sciences
    Volume368
    Issue number1620
    DOIs
    Publication statusPublished - 19 Jun 2013

    Keywords

    • α-globin locus
    • Chromatin conformation capture
    • cis-regulatory elements

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    Hughes, J., Lower, K., Dunham, I., Taylor, S., De Gobbi, M., Sloane-Stanley, J., McGowan, S., Ragoussis, J., Vernimmen, D., Gibbons, R., & Higgs, D. (2013). High-resolution analysis of cis-acting regulatory networks at the α-globin locus. Philosophical Transactions of the Royal Society of London Series B: Biological Sciences, 368(1620), 20120361. https://doi.org/10.1098/rstb.2012.0361