TY - JOUR
T1 - High-resolution analysis of cis-acting regulatory networks at the α-globin locus
AU - Hughes, Jim
AU - Lower, Karen
AU - Dunham, Ian
AU - Taylor, Stephen
AU - De Gobbi, Marco
AU - Sloane-Stanley, Jackie
AU - McGowan, Simon
AU - Ragoussis, Jiannis
AU - Vernimmen, Douglas
AU - Gibbons, Richard
AU - Higgs, Doug
PY - 2013/6/19
Y1 - 2013/6/19
N2 - We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the a-globin cluster.
AB - We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the a-globin cluster.
KW - α-globin locus
KW - Chromatin conformation capture
KW - cis-regulatory elements
UR - http://www.scopus.com/inward/record.url?scp=84877124067&partnerID=8YFLogxK
U2 - 10.1098/rstb.2012.0361
DO - 10.1098/rstb.2012.0361
M3 - Article
SN - 0962-8436
VL - 368
SP - 20120361
JO - Philosophical Transactions of the Royal Society of London Series B: Biological Sciences
JF - Philosophical Transactions of the Royal Society of London Series B: Biological Sciences
IS - 1620
ER -