Hindered protein dynamics in the presence of a cryoprotecting agent

I. Köper, M. C. Bellissent-Funel

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


We present a study of the influence of trehalose, a well-known cryoprotecting disaccharide, on the dynamics of a protein, the C-phycocyanin. The dynamics is investigated in a time range from some picoseconds to several nanoseconds using different neutron-scattering techniques. Data obtained on samples containing hydrated powders of the protein in the presence of trehalose are compared to that of the protein alone, studied by neutron-scattering techniques as well as by molecular dynamics simulations. The analysis of time-of-flight data gives access to the geometry of the observed motions. These motions can be described via a model of a particle diffusing inside a sphere. We observe a slowing down of the movements of the protein due to the presence of trehalose of one to two orders of magnitude, while the geometry of the motions is conserved.

Original languageEnglish
Pages (from-to)s1257-s1259
Number of pages3
JournalApplied Physics A: Materials Science and Processing
Issue numberSuppl.
Publication statusPublished - Dec 2002
Externally publishedYes


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