Human amniotic fluid stem cells possess the potential to differentiate into primordial follicle oocytes in vitro

Xiaoli Yu, Ning Wang, Rong Qiang, Qianhui Wan, Mingming Qin, Shuai Chen, Huayan Wang

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Previous reports have demonstrated that embryonic stem cells were capable of differentiating into primordial germ cells through the formation of embryoid bodies that subsequently generated oocyte-like cells (OLCs). Such a process could facilitate studies of primordial follicle oocyte development in vitro and regenerative medicine. To investigate the pluripotency of human amniotic fluid stem cells (hAFSCs) and their ability to differentiate into germ cells, we isolated a CD117+/CD44+ hAFSC line that showed fibroblastoid morphology and intrinsically expressed both stem cell markers (OCT4, NANOG, SOX2) and germ cell markers (DAZL, STELLA). To encourage differentiation into OLCs, the hAFSCs were first cultured in a medium supplemented with 5% porcine follicular fluid for 10 days. During the induction period, cell aggregates formed and syntheses of steroid hormones were detected; some OLCs and granulosa cell-like cells could be loosened from the surface of the culture dish. Cell aggregates were collected and replated in oocyte culture medium for an additional 7-10 days. OLCs ranging from 50 to 120 μm presenting zona pellucida were observed in cumulus-oocyte complexes; some OLCs developed spontaneously into multicell structures similar to preimplantation embryos. Approximately 2% of the hAFSCs differentiated to meiotic germ cells that expressed folliculogenesis- and oogenesis-associated markers. Although the in vitro maturation and fertilization potentials are as yet unproven, short-term (< 25 days) and high-efficiency (> 2%) derivation of OLCs from hAFSCs might provide a new approach to the study of human germ cell development in vitro.

Original languageEnglish
Article number73
Number of pages11
JournalBiology of Reproduction
Volume90
Issue number4
DOIs
Publication statusPublished - 1 Apr 2014
Externally publishedYes

Keywords

  • Differentiation
  • Embryo
  • Human reproduction
  • Oocyte maturation
  • Stem cells

Fingerprint

Dive into the research topics of 'Human amniotic fluid stem cells possess the potential to differentiate into primordial follicle oocytes in vitro'. Together they form a unique fingerprint.

Cite this