TY - JOUR
T1 - Hydrogel-Film-Fabricated Fluorescent Biosensors with Aggregation-Induced Emission for Albumin Detection through the Real-Time Modulation of a Vortex Fluidic Device
AU - Hu, Qi
AU - Luo, Xuan
AU - Tohl, Damian
AU - Pham, Anh Tran Tam
AU - Raston, Colin
AU - Tang, Youhong
PY - 2023/4/1
Y1 - 2023/4/1
N2 - Hydrogels have various promising prospects as a successful platform for detecting biomarkers, and human serum albumin (HSA) is an important biomarker in the diagnosis of kidney diseases. However, the difficult-to-control passive diffusion kinetics of hydrogels is a major factor affecting detection performance. This study focuses on using hydrogels embedded with aggregation-induced emission (AIE) fluorescent probe TC426 to detect HSA in real time. The vortex fluidic device (VFD) technology is used as a rotation strategy to control the reaction kinetics and micromixing during measurement. The results show that the introduction of VFD could significantly accelerate its fluorescence response and effectively improve the diffusion coefficient, while VFD processing could regulate passive diffusion into active diffusion, offering a new method for future sensing research.
AB - Hydrogels have various promising prospects as a successful platform for detecting biomarkers, and human serum albumin (HSA) is an important biomarker in the diagnosis of kidney diseases. However, the difficult-to-control passive diffusion kinetics of hydrogels is a major factor affecting detection performance. This study focuses on using hydrogels embedded with aggregation-induced emission (AIE) fluorescent probe TC426 to detect HSA in real time. The vortex fluidic device (VFD) technology is used as a rotation strategy to control the reaction kinetics and micromixing during measurement. The results show that the introduction of VFD could significantly accelerate its fluorescence response and effectively improve the diffusion coefficient, while VFD processing could regulate passive diffusion into active diffusion, offering a new method for future sensing research.
KW - aggregation-induced emission
KW - fluorescence biosensor
KW - human serum albumin
KW - hydrogel films
KW - vortex fluidic device
UR - http://www.scopus.com/inward/record.url?scp=85152322668&partnerID=8YFLogxK
U2 - 10.3390/molecules28073244
DO - 10.3390/molecules28073244
M3 - Article
C2 - 37050007
AN - SCOPUS:85152322668
SN - 1420-3049
VL - 28
JO - Molecules
JF - Molecules
IS - 7
M1 - 3244
ER -
