Identification and quantification of mRNA for nerve growth factor in histological preparations

P. A. Wilson; J. Scott; J. Penschow; J. Coghlan; R. A. Rush

doi:10.1016/0304-3940(86)90349-6

Identification and quantification of mRNA for nerve growth factor in histological preparations

P. A. Wilson, J. Scott, J. Penschow, J. Coghlan, R. A. Rush

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13 Citations (Scopus)

Abstract

Hybridization histochemistry has been used to detect mRNA for nerve growth factor (NGF) in histological preparations of mouse salivary glands and rat iris using a ³²P-labelled cDNA probe and autoradiography. Label was visible over the tubular cells of the male mouse submaxillary gland but not the sublingual gland. A much lower label density was found over the tubular cells of the female submaxillary gland, whereas sections of liver and pancreas were negative. Quantitative autoradiography allowed the detection of low levels of mRNA for NGF in the rat iris which was elevated by prior culture of the tissue. The results provide direct histological evidence for the presence of specific NGF-mRNA in the mouse submaxillary gland and rat iris, with increased levels following culture.

Original language	English
Pages (from-to)	323-329
Number of pages	7
Journal	Neuroscience letters
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(86)90349-6
Publication status	Published - 14 Mar 1986

Keywords

complementary DNA
hybridization histochemistry
mouse salivary gland
rat iris

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title = "Identification and quantification of mRNA for nerve growth factor in histological preparations",

abstract = "Hybridization histochemistry has been used to detect mRNA for nerve growth factor (NGF) in histological preparations of mouse salivary glands and rat iris using a 32P-labelled cDNA probe and autoradiography. Label was visible over the tubular cells of the male mouse submaxillary gland but not the sublingual gland. A much lower label density was found over the tubular cells of the female submaxillary gland, whereas sections of liver and pancreas were negative. Quantitative autoradiography allowed the detection of low levels of mRNA for NGF in the rat iris which was elevated by prior culture of the tissue. The results provide direct histological evidence for the presence of specific NGF-mRNA in the mouse submaxillary gland and rat iris, with increased levels following culture.",

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T1 - Identification and quantification of mRNA for nerve growth factor in histological preparations

AU - Wilson, P. A.

AU - Scott, J.

AU - Penschow, J.

AU - Coghlan, J.

AU - Rush, R. A.

PY - 1986/3/14

Y1 - 1986/3/14

N2 - Hybridization histochemistry has been used to detect mRNA for nerve growth factor (NGF) in histological preparations of mouse salivary glands and rat iris using a 32P-labelled cDNA probe and autoradiography. Label was visible over the tubular cells of the male mouse submaxillary gland but not the sublingual gland. A much lower label density was found over the tubular cells of the female submaxillary gland, whereas sections of liver and pancreas were negative. Quantitative autoradiography allowed the detection of low levels of mRNA for NGF in the rat iris which was elevated by prior culture of the tissue. The results provide direct histological evidence for the presence of specific NGF-mRNA in the mouse submaxillary gland and rat iris, with increased levels following culture.

AB - Hybridization histochemistry has been used to detect mRNA for nerve growth factor (NGF) in histological preparations of mouse salivary glands and rat iris using a 32P-labelled cDNA probe and autoradiography. Label was visible over the tubular cells of the male mouse submaxillary gland but not the sublingual gland. A much lower label density was found over the tubular cells of the female submaxillary gland, whereas sections of liver and pancreas were negative. Quantitative autoradiography allowed the detection of low levels of mRNA for NGF in the rat iris which was elevated by prior culture of the tissue. The results provide direct histological evidence for the presence of specific NGF-mRNA in the mouse submaxillary gland and rat iris, with increased levels following culture.

KW - complementary DNA

KW - hybridization histochemistry

KW - mouse salivary gland

KW - rat iris

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VL - 64

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JO - Neuroscience letters

JF - Neuroscience letters

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Identification and quantification of mRNA for nerve growth factor in histological preparations

Abstract

Keywords

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