TY - JOUR
T1 - Identification and validation of circulating miRNAs as endogenous controls in obstructive sleep apnea
AU - Santamaria-Martos, Fernando
AU - Benítez, Ivan
AU - Zapater, Andrea
AU - Girón, Cristina
AU - Pinilla, Lucía
AU - Fernandez-Real, Jose Manuel
AU - Barbé, Ferran
AU - Ortega, Francisco Jose
AU - Sánchez-de-La-Torre, Manuel
PY - 2019/3/13
Y1 - 2019/3/13
N2 - microRNAs (miRNAs) are non-coding RNAs highly relevant as biomarkers for disease. A seminal study that explored the role of miRNAs in obstructive sleep apnea syndrome (OSA) demonstrated their usefulness in clinical management. Nevertheless, the miRNAs that may act as endogenous controls (ECs) have not yet been established. The identification of ECs would contribute to the standardization of these biomarkers in OSA. The objective of the study is to identify miRNAs that can be used as ECs in OSA. We evaluated 100 patients divided into two different cohorts: a learning cohort of 10 non-OSA and 30 OSA patients, and a validation cohort (20 non-OSA and 40 OSA patients). In the learning cohort, a profile of 188 miRNAs was determined in plasma by TaqMan Low Density Array. The best EC candidates were identified by mean center+SD normalization and concordance correlation restricted normalization. The results were validated using NormFinder and geNorm to assess the stability of those ECs. Eight miRNAs were identified as EC candidates. The combination miRNA-106a/miRNA-186 was identified as the most stable among all candidates. We identified a set of ECs to be used in the determination of circulating miRNA in OSA that may contribute to the homogeneity of results.
AB - microRNAs (miRNAs) are non-coding RNAs highly relevant as biomarkers for disease. A seminal study that explored the role of miRNAs in obstructive sleep apnea syndrome (OSA) demonstrated their usefulness in clinical management. Nevertheless, the miRNAs that may act as endogenous controls (ECs) have not yet been established. The identification of ECs would contribute to the standardization of these biomarkers in OSA. The objective of the study is to identify miRNAs that can be used as ECs in OSA. We evaluated 100 patients divided into two different cohorts: a learning cohort of 10 non-OSA and 30 OSA patients, and a validation cohort (20 non-OSA and 40 OSA patients). In the learning cohort, a profile of 188 miRNAs was determined in plasma by TaqMan Low Density Array. The best EC candidates were identified by mean center+SD normalization and concordance correlation restricted normalization. The results were validated using NormFinder and geNorm to assess the stability of those ECs. Eight miRNAs were identified as EC candidates. The combination miRNA-106a/miRNA-186 was identified as the most stable among all candidates. We identified a set of ECs to be used in the determination of circulating miRNA in OSA that may contribute to the homogeneity of results.
KW - microRNAs
KW - biomarkers
KW - blood plasma
KW - reverse transcriptase-polymerase chain reaction
KW - sleep apnea
KW - algorithms
KW - oxygen
KW - non-coding RNA
UR - http://www.scopus.com/inward/record.url?scp=85062831781&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0213622
DO - 10.1371/journal.pone.0213622
M3 - Article
C2 - 30865706
AN - SCOPUS:85062831781
SN - 1932-6203
VL - 14
JO - PLoS One
JF - PLoS One
IS - 3
M1 - e0213622
ER -