Identification of an inversion 16 coexisting with an isochromosome 22q by in situ hybridization in a case of childhood AML M4e

Sonia G. Gad, David F. Gallen, Bryone Kuss, James R. Downing, Frederick Behm, David Head, Raul C. Ribeiro, Susana C. Raimondi

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Abstract

Rearrangements involving chromosome 16, including inv(16) (p13q22), del(16)(q22), and t(16;16) (p13;q22), are frequent findings in acute myeloblastic leukemia (AML). Each of these rearrangements can occur as the sole karyotypic change or in association with additional chromosomal abnormalities, including in decreasing order of frequency: trisomy 22, trisomy 8, and deletion of the long arm of chromosome 7. We report a pediatric case of de novo AML, M4e subtype, with a unique combination of inv(16) (p13q22) and i(22q) occurring within the same leukemic clone. The inv(16) was detected by fluorescence in situ hybridization (FISH) analysis with two cosmid probes specific for sequences flanking the inv(16) breakpoint on the long arm of chromosome 16. Use of a chromosome-22-specific painting probe unequivocally identified a small metacentric chromosome as an i(22q). This case illustrates a variation in the association of trisomy 22 with inv(16) and suggests that duplication of the long arm of chromosome 22 may contain critical gene(s) involved in the multistep process of evolution of leukemia with 16q22 abnormalities.

Original languageEnglish
Pages (from-to)1658-1662
Number of pages5
JournalLeukemia
Volume7
Issue number10
Publication statusPublished - 1 Oct 1993
Externally publishedYes

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    Gad, S. G., Gallen, D. F., Kuss, B., Downing, J. R., Behm, F., Head, D., Ribeiro, R. C., & Raimondi, S. C. (1993). Identification of an inversion 16 coexisting with an isochromosome 22q by in situ hybridization in a case of childhood AML M4e. Leukemia, 7(10), 1658-1662. https://www.ncbi.nlm.nih.gov/pubmed/8412329