Identification of Fibroblast Growth Factors in Bovine Cheese Whey

Mary Louise Rogers, David A. Belford, Geoffrey L. Francis, F. John Ballard

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin—sepharose column and eluted stepwise with 0.8, 1.2 and 2.0 m-nh 4 HCO3. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1–2 M-eluate and basic FGF in the 2.0 M-eluate, but neither acidic nor basic FGF was detected in the 0.8 M-fraction. Quantitative radioreceptor assays indicated 5.8 ng of acidic FGF-like activity and 19.8 ng of basic FGF-like activity perl whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.

Original languageEnglish
Pages (from-to)501-507
Number of pages7
JournalJOURNAL OF DAIRY RESEARCH
Volume62
Issue number3
DOIs
Publication statusPublished - Aug 1995
Externally publishedYes

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