Identification of Fibroblast Growth Factors in Bovine Cheese Whey

Mary Louise Rogers; David A. Belford; Geoffrey L. Francis; F. John Ballard

doi:10.1017/S0022029900031198

Identification of Fibroblast Growth Factors in Bovine Cheese Whey

Mary Louise Rogers, David A. Belford, Geoffrey L. Francis, F. John Ballard

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32 Citations (Scopus)

Abstract

Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin—sepharose column and eluted stepwise with 0.8, 1.2 and 2.0 m-nh 4 HCO₃. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1–2 M-eluate and basic FGF in the 2.0 M-eluate, but neither acidic nor basic FGF was detected in the 0.8 M-fraction. Quantitative radioreceptor assays indicated 5.8 ng of acidic FGF-like activity and 19.8 ng of basic FGF-like activity perl whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.

Original language	English
Pages (from-to)	501-507
Number of pages	7
Journal	JOURNAL OF DAIRY RESEARCH
Volume	62
Issue number	3
DOIs	https://doi.org/10.1017/S0022029900031198
Publication status	Published - Aug 1995
Externally published	Yes

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title = "Identification of Fibroblast Growth Factors in Bovine Cheese Whey",

abstract = "Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin—sepharose column and eluted stepwise with 0.8, 1.2 and 2.0 m-nh 4 HCO3. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1–2 M-eluate and basic FGF in the 2.0 M-eluate, but neither acidic nor basic FGF was detected in the 0.8 M-fraction. Quantitative radioreceptor assays indicated 5.8 ng of acidic FGF-like activity and 19.8 ng of basic FGF-like activity perl whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.",

author = "Rogers, {Mary Louise} and Belford, {David A.} and Francis, {Geoffrey L.} and Ballard, {F. John}",

year = "1995",

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T1 - Identification of Fibroblast Growth Factors in Bovine Cheese Whey

AU - Rogers, Mary Louise

AU - Belford, David A.

AU - Francis, Geoffrey L.

AU - Ballard, F. John

PY - 1995/8

Y1 - 1995/8

N2 - Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin—sepharose column and eluted stepwise with 0.8, 1.2 and 2.0 m-nh 4 HCO3. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1–2 M-eluate and basic FGF in the 2.0 M-eluate, but neither acidic nor basic FGF was detected in the 0.8 M-fraction. Quantitative radioreceptor assays indicated 5.8 ng of acidic FGF-like activity and 19.8 ng of basic FGF-like activity perl whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.

AB - Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin—sepharose column and eluted stepwise with 0.8, 1.2 and 2.0 m-nh 4 HCO3. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1–2 M-eluate and basic FGF in the 2.0 M-eluate, but neither acidic nor basic FGF was detected in the 0.8 M-fraction. Quantitative radioreceptor assays indicated 5.8 ng of acidic FGF-like activity and 19.8 ng of basic FGF-like activity perl whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.

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Identification of Fibroblast Growth Factors in Bovine Cheese Whey

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Modified milk growth factor

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