Identification of motor neurons to the longitudinal muscle of the guinea pig ileum

S. J.H. Brookes, Z. M. Song, P. A. Steele, M. Costa

    Research output: Contribution to journalArticlepeer-review

    99 Citations (Scopus)

    Abstract

    Motor neurons that innervate the longitudinal muscle of the guinea pig ileum were identified by retrograde transport from the longitudinal muscle plexus in organotypic culture. Motor neurons had short projections, <3.5 mm long, and never had Dogiel type II morphology; most labeled neurons had morphological characteristics of Dogiel type I neurons. Immunoreactivity for choline acetyltransferase was present in 97% of retrogradely labeled nerve cell bodies, reflecting the dominant cholinergic input to the longitudinal muscle layer. Substance P immunoreactivity was present in 48% of motor neurons, indicating that it or a similar tachykinin that mediates noncholinergic excitatory transmission is likely to be released by a subset of cholinergic motor neurons. This strongly suggests that the difference in frequency dependence of substance P and acetylcholine release is attributable to different release mechanisms rather than to activation of separate populations of motor neurons. Immunoreactivity for the calcium-binding protein calretinin was present in 87% of longitudinal muscle motor neurons. The neurochemical coding of longitudinal muscle motor neurons indicated that they constitute about one quarter of all myenteric neurons and are distinct from circular muscle motor neurons.

    Original languageEnglish
    Pages (from-to)961-973
    Number of pages13
    JournalGastroenterology
    Volume103
    Issue number3
    DOIs
    Publication statusPublished - Sep 1992

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