Identification of the Main Intermediate Precursor of L-Ergothioneine Biosynthesis in Human Biological Specimens

Salvatore Sotgia, Arduino Mangoni, Mauro Forteschi, Rhys Murphy, David Elliot, Elisabetta Sotgiu, Gianfranco Pintus, Ciriaco Carru, Angelo Zinellu

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    A capillary electrophoresis coupled to tandem mass spectrometry (CE-MS/MS) has been used to make a qualitative determination of hercynine - the main precursor of L-ergothioneine biosynthesis - in some key human biological specimens, such as urine, whole blood, plasma, and saliva. From semiquantitative analysis results, the highest concentrations of hercynine were detected in saliva and whole blood, whereas much lower concentrations were measured in urine and plasma. Whole blood was the biological matrix with the highest concentration of L-ergothioneine followed by plasma, saliva, and urine. The antioxidant effects attributed to L-ergothioneine, along with its peculiar antioxidant mechanism, offer a possible explanation for the presence of the hercynine, as well as its concentration, in the considered biological matrices.

    Original languageEnglish
    Article number1298
    Pages (from-to)Art: 1298
    Number of pages7
    JournalMolecules
    Volume21
    Issue number10
    DOIs
    Publication statusPublished - 1 Oct 2016

    Keywords

    • Aminothione
    • Capillary electrophoresis
    • Dismutation
    • Mass spectrometry
    • Quaternary ammonium

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