Immobilization of β-glucosidase on a magnetic nanoparticle improves thermostability: Application in cellobiose hydrolysis

Madan Verma, Rajneesh Chaudhary, Takuya Tsuzuki, Colin Barrow, Munish Puri

    Research output: Contribution to journalArticlepeer-review

    100 Citations (Scopus)

    Abstract

    The objective of the present work was to develop a thermostable β-glucosidase through immobilization on a nanoscale carrier for potential application in biofuel production. β-Glucosidase (BGL) from Aspergillus niger was immobilized to functionalized magnetic nanoparticles by covalent binding. Immobilized nanoparticles showed 93% immobilization binding. Immobilized and free BGL were characterized using Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) techniques. Free and immobilized enzyme exhibited different pH-optima at pH 4.0 and 6.0, respectively, but had the same temperature optima at 60°C. Michaelis constant (K M) was 3.5 and 4.3mM for free and immobilized BGL. Thermal stability of the immobilized enzyme was enhanced at 70°C. The immobilized nanoparticle-enzyme conjugate retained more than 50% enzyme activity up to the 16th cycle. Maximum glucose synthesis from cellobiose hydrolysis by immobilized BGL was achieved at 16h.

    Original languageEnglish
    Pages (from-to)2-6
    Number of pages5
    JournalBioresource Technology
    Volume135
    DOIs
    Publication statusPublished - May 2013

    Keywords

    • Biofuel
    • Cellulase
    • Iron oxide
    • Lignocellulose
    • Nanobiotechnology

    Fingerprint Dive into the research topics of 'Immobilization of β-glucosidase on a magnetic nanoparticle improves thermostability: Application in cellobiose hydrolysis'. Together they form a unique fingerprint.

    Cite this