Immuno- and affinity probes for electron microscopy: A review of labeling and preparation techniques

J. W. Stirling

Research output: Contribution to journalReview articlepeer-review

84 Citations (Scopus)

Abstract

Immuno- and affinity probes are widely used in biology and medicine, and are becoming essential tools for the elucidation of cell structure and function. This article reviews and discusses the bewildering array of probes and preparation techniques now available for the investigation of sectioned material by transmission electron microscopy, with critical analysis of their merits. Emphasis is placed on immunogold probes and methods useful for routine preparation, gathering together information that may be used to improve labeling techniques. New data on inert dehydration for the localization of sensitive epitopes without chemical or cryofixation is presented.

Original languageEnglish
Pages (from-to)145-157
Number of pages13
JournalJournal of Histochemistry and Cytochemistry
Volume38
Issue number2
DOIs
Publication statusPublished - 1 Feb 1990
Externally publishedYes

Bibliographical note

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Affinity labeling
  • Electron microscopy
  • Immunogold labeling
  • Inert dehydration
  • Preparation techniques

Fingerprint

Dive into the research topics of 'Immuno- and affinity probes for electron microscopy: A review of labeling and preparation techniques'. Together they form a unique fingerprint.

Cite this