Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis

Kym McNicholas, Michael Z Michael

    Research output: Chapter in Book/Report/Conference proceedingChapter

    11 Citations (Scopus)

    Abstract

    Due to their size, small extracellular vesicles such as exosomes have been difficult to identify and to quantify. As the roles that exosomes play in intercellular signalling become clearer, so does their potential utility as both diagnostic biomarkers for disease and as therapeutic vectors. Accurate assessment of exosomes, both their number and their cargo, is important for continued advancement in the field of vesicle research. To that end, several technologies, including nanoparticle tracking analysis, have been developed to define the physical characteristics of vesicle preparations and determine their concentration. This chapter describes a method for identifying the size and concentration of a subpopulation of vesicles in biological samples, using nanoparticle tracking analysis. Characterization of distinct exosomes is enabled by specific marker antibodies, coupled to fluorescent quantum dots.

    Original languageEnglish
    Title of host publicationExosomes and microvesicles
    Subtitle of host publicationmethods and protocols
    EditorsAndrew F Hill
    Place of PublicationNew York
    PublisherHUMANA PRESS
    Pages35-42
    Number of pages8
    Volume1545
    ISBN (Electronic)9781493967285
    ISBN (Print)9781493967261
    DOIs
    Publication statusPublished - 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1545
    ISSN (Print)1064-3745

    Keywords

    • quantum dots
    • exosomes
    • NanoSight

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