Abstract
This paper describes the immunohistochemical techniques which can be used to detect cytokines and cell adhesion molecules in synovial membrane tissue, including a list of reagents and possible problems in each technique. It also describes three methods of quantitation of the resultant immunohistochemical detection, including the recent innovation computer- assisted digital video image analysis, and lists the advantages and disadvantages of each quantitation technique. This information will be a useful summary for any scientist interested in applying such techniques to the detection of cytokines and cell adhesion molecules in human tissue sections.
Original language | English |
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Pages (from-to) | 311-319 |
Number of pages | 9 |
Journal | Methods and Findings in Experimental and Clinical Pharmacology |
Volume | 21 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1999 |
Keywords
- Cell adhesion molecules
- Cytokines
- Immunohistochemistry
- Quantitation