Impact of Dietary Vitamin D3Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

Shubin Liu, Xiaodan Wang, Xianyong Bu, Zhideng Lin, Erchao Li, Qingchao Shi, Meiling Zhang, Jian G. Qin, Liqiao Chen

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Vitamin D3 (vit-D3), as an indispensable and fat-soluble nutrient, is associated with skeletal mineralization and health in mammals. However, such associations have not been well studied in economically important crustaceans. Six levels of vit-D3 with isonitrogenous and isolipidic diets were used to feed Eriocheir sinensis. The range of optimal vit-D3 requirements is 5685.43-10,000 IU/kg based on growth. The crabs fed 9000 IU/kg vit-D3 showed the best growth performance. This vit-D3 dose significantly increased antioxidant capacity in the hepatopancreas and intestine and was optimal for molting and innate immunity via quantitative polymerase chain reaction analysis. Transcriptomics analyses indicate that vit-D3 could alter protein processing in the endoplasmic reticulum, steroid biosynthesis, and antigen processing and presentation. As shown by the enzyme-linked immunosorbent assay, vit-D3 could improve vitamin D receptor, retinoic acid receptor, and C-type lectins concentrations. The 1α,25-dihydroxy vit-D3 content in serum was significantly higher in 3000-9000 IU/kg vit-D3. The study suggests that dietary vit-D3 and its metabolites can regulate molting and innate immunity in crabs.

Original languageEnglish
Pages (from-to)12794-12806
Number of pages13
JournalJournal of Agricultural and Food Chemistry
Volume69
Issue number43
DOIs
Publication statusPublished - 3 Nov 2021

Keywords

  • Eriocheir sinensis
  • hepatopancreas
  • innate immunity
  • intestine
  • molting
  • vit-D

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