Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression

Robert A. Edwards, Linda H. Keller, Dieter M. Schifferli

Research output: Contribution to journalArticlepeer-review

382 Citations (Scopus)

Abstract

A series of vectors has been developed to provide improved positive and negative selection for allelic exchange. Based on homologous regions of DNA ranging in size from less than 200 bp to over 1 kb, we have successfully used these new plasmids to introduce or remove markers in chromosomal or plasmid DNA. Wild type fimbria genes were replaced both in Salmonella enteritidis (sefA, agfA and fimC) and Escherichia coli (fasA and fasH). Regulation of 987P fimbriation could be identified after replacement of fasA and fasH with allelic reporter fusions. The expression of fasA but not fasH is dependent upon the osmolarity of the growth medium in an HNS-dependent manner, but unlike some other fimbrial systems expression is not dependent on the exogenous iron concentration.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalGene
Volume207
Issue number2
DOIs
Publication statusPublished - 30 Jan 1998
Externally publishedYes

Keywords

  • 987P
  • Fimbriae
  • hns
  • Osmolarity
  • Pili
  • sacB

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