TY - JOUR
T1 - Improved immunohistochemical visualization of central serotonin nerves after loading with 5,7-dihydroxytryptamine
AU - Howe, P. R.C.
AU - Cuello, A. C.
AU - Costa, M.
AU - Furness, J. B.
PY - 1982/3/17
Y1 - 1982/3/17
N2 - 5,7-Dihydroxytryptamine (5,7-DHT), which reacts with a monoclonal antibody raised against 5-hydroxytryptamine (5-HT), has been used to increase the sensitivity of the immunohistochemical detection by this antibody of central 5-HT nerves in the rat brain. Rats pretreated with tricyclic antidepressants were given a single intraventricular injection of 5,7-DHT and were sacrificed 30 min-3 h later. This treatment resulted in accumulation of 5,7-DHT by central 5-HT nerves and selectively enhanced the immunofluorescence reaction of these nerves, particularly the terminals and non-terminal fibres.
AB - 5,7-Dihydroxytryptamine (5,7-DHT), which reacts with a monoclonal antibody raised against 5-hydroxytryptamine (5-HT), has been used to increase the sensitivity of the immunohistochemical detection by this antibody of central 5-HT nerves in the rat brain. Rats pretreated with tricyclic antidepressants were given a single intraventricular injection of 5,7-DHT and were sacrificed 30 min-3 h later. This treatment resulted in accumulation of 5,7-DHT by central 5-HT nerves and selectively enhanced the immunofluorescence reaction of these nerves, particularly the terminals and non-terminal fibres.
UR - http://www.scopus.com/inward/record.url?scp=84950170834&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(82)90354-8
DO - 10.1016/0304-3940(82)90354-8
M3 - Article
C2 - 7041011
AN - SCOPUS:84950170834
VL - 29
SP - 1
EP - 6
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -