Improved immunohistochemical visualization of central serotonin nerves after loading with 5,7-dihydroxytryptamine

P. R.C. Howe, A. C. Cuello, M. Costa, J. B. Furness

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    15 Citations (Scopus)

    Abstract

    5,7-Dihydroxytryptamine (5,7-DHT), which reacts with a monoclonal antibody raised against 5-hydroxytryptamine (5-HT), has been used to increase the sensitivity of the immunohistochemical detection by this antibody of central 5-HT nerves in the rat brain. Rats pretreated with tricyclic antidepressants were given a single intraventricular injection of 5,7-DHT and were sacrificed 30 min-3 h later. This treatment resulted in accumulation of 5,7-DHT by central 5-HT nerves and selectively enhanced the immunofluorescence reaction of these nerves, particularly the terminals and non-terminal fibres.

    Original languageEnglish
    Pages (from-to)1-6
    Number of pages6
    JournalNeuroscience letters
    Volume29
    Issue number1
    DOIs
    Publication statusPublished - 17 Mar 1982

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