Improved immunohistochemical visualization of central serotonin nerves after loading with 5,7-dihydroxytryptamine

P. R.C. Howe; A. C. Cuello; M. Costa; J. B. Furness

doi:10.1016/0304-3940(82)90354-8

Improved immunohistochemical visualization of central serotonin nerves after loading with 5,7-dihydroxytryptamine

P. R.C. Howe, A. C. Cuello, M. Costa, J. B. Furness

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Abstract

5,7-Dihydroxytryptamine (5,7-DHT), which reacts with a monoclonal antibody raised against 5-hydroxytryptamine (5-HT), has been used to increase the sensitivity of the immunohistochemical detection by this antibody of central 5-HT nerves in the rat brain. Rats pretreated with tricyclic antidepressants were given a single intraventricular injection of 5,7-DHT and were sacrificed 30 min-3 h later. This treatment resulted in accumulation of 5,7-DHT by central 5-HT nerves and selectively enhanced the immunofluorescence reaction of these nerves, particularly the terminals and non-terminal fibres.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Neuroscience letters
Volume	29
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(82)90354-8
Publication status	Published - 17 Mar 1982

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abstract = "5,7-Dihydroxytryptamine (5,7-DHT), which reacts with a monoclonal antibody raised against 5-hydroxytryptamine (5-HT), has been used to increase the sensitivity of the immunohistochemical detection by this antibody of central 5-HT nerves in the rat brain. Rats pretreated with tricyclic antidepressants were given a single intraventricular injection of 5,7-DHT and were sacrificed 30 min-3 h later. This treatment resulted in accumulation of 5,7-DHT by central 5-HT nerves and selectively enhanced the immunofluorescence reaction of these nerves, particularly the terminals and non-terminal fibres.",

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AU - Furness, J. B.

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Improved immunohistochemical visualization of central serotonin nerves after loading with 5,7-dihydroxytryptamine

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