Improvement in non-programmable sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata

Yibing Liu, Xiaoxu Li, T Xu, Nicholas Robinson, Jianguang Qin

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

This study assessed the effects of the addition of vitamins (. l-ascorbic acid), amino acids (glycine and taurine), and monosaccharides (glucose, fructose and galactose) on sperm cryopreservation using a non-programmable freezing technique in farmed greenlip abalone. The results showed that the addition of taurine, glycine or l-ascorbic acid significantly improved the post-thaw sperm motility, whereas the post-thaw sperm fertilization rates were improved by the addition of glycine or l-ascorbic acid. Flow cytometry analysis demonstrated that the addition of glycine significantly enhanced the post-thaw sperm plasma membrane integrity and acrosome integrity. Results from the investigation on monosaccharides demonstrated that glucose, fructose and galactose had a similar cryoprotective effect, resulting in a similar level of the post-thaw sperm fertilization rate, plasma membrane integrity, mitochondrial membrane potential and acrosome integrity. In this study, the highest post-thaw fertilization rate of 96% was achieved by using the cryoprotective mediums containing 6% DMSO, 1% glucose and 0.6% glycine.

Original languageEnglish
Pages (from-to)362-366
Number of pages5
JournalAquaculture
Volume434
DOIs
Publication statusPublished - 20 Oct 2014

Keywords

  • Amino acid
  • Farmed greenlip abalone
  • Monosaccharides
  • Non-programmable freezing technique
  • Vitamin

Fingerprint Dive into the research topics of 'Improvement in non-programmable sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata'. Together they form a unique fingerprint.

Cite this