In vitro and in vivo evaluation of probiotic properties of Corynebacterium accolens isolated from the human nasal cavity

Martha Alemayehu Menberu, Clare Cooksley, Mahnaz Ramezanpour, George Bouras, Peter John Wormald, Alkis James Psaltis, Sarah Vreugde

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Corynebacterium accolens strains are increasingly recognized as beneficial bacteria that can confer a health benefit on the host. In the current study, the probiotic potential of three C. accolens strains, C779, C781 and C787 derived from a healthy human nasal cavity were investigated. These strains were examined for their adhesion to HNECs, competition with Staphylococcus aureus for adhesion, toxicity, induction of IL-6, antibiotic susceptibility and the presence of antibiotic resistance and virulence genes. Furthermore, the safety and efficacy of strains were evaluated in vivo using Caenorhabditis elegans. The adhesion capacity of C. accolens to HNECs was strain-dependent. Highest adhesion was observed for strain C781. None of the C. accolens strains tested caused cell lysis. All strains were able to outcompete S. aureus for cell adhesion and caused a significant decrease of IL-6 production by HNECs co-exposed to S. aureus when compared to the control groups. All strains were sensitive or showed intermediate sensitivity to 10 different antibiotics. Whole Genome Sequence analysis showed C. accolens C781 and C787 did not possess antibiotic resistance genes whereas strain C779 harboured 5 genes associated with resistance to Aminoglycoside, Chloramphenicol and Erythromycin. In addition, no virulence genes were detected in any of the 3 strains. Moreover, the tested strains had no detrimental effect on worm survival and induced protection from S. aureus-mediated infection. Taken all together, C. accolens strains, C781 and C787 displayed probiotic potential and hold promise for use in clinical applications for combating dysbiosis in chronic rhinosinusitis.

Original languageEnglish
Article number126927
Number of pages10
JournalMicrobiological Research
Volume255
Early online date23 Nov 2021
DOIs
Publication statusPublished - Feb 2022
Externally publishedYes

Keywords

  • Chronic rhinosinusitis
  • Corynebacteriumaccolens
  • Dysbiosis
  • Human nasal cavity
  • Probiotic

Fingerprint

Dive into the research topics of 'In vitro and in vivo evaluation of probiotic properties of Corynebacterium accolens isolated from the human nasal cavity'. Together they form a unique fingerprint.

Cite this