In vitro evaluation of methanol extract of Agrimonia eupatoria on PBMC and 12 pathogenic bacteria

M. S. Masjedi, M. Keyhanfar, M. Behbahani

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1 Citation (Scopus)


Background: Today the use of medicinal plants to improve the immune system function and against pathogenic bacteria has been considered. Objective: In this study, the effect of methanol extract of the aerial parts of Agrimonia eupatoria on peripheral blood mononuclear cells (PBMC) and 12 pathogenic bacteria were investigated. Methods: The methanol extract of branches, stems, seeds and leaves of Agrimonia eupatoria were prepared and the effect of different concentrations of 50, 100, 500, 1000, 1500, 2000 and 2500 Hg/ml of the extract on proliferation of PBMC was evaluated by MTT assay. Also the effect of concentrations of 1, 2, 4, 5, 7 and 10 mg/ml of the extract was tested on 12 pathogenic bacteria by disc method and on nutrient agar media. Results: The methanol extract of the branch, stem and seed of Agrimonia eupatoria showed the most stimulatory effects on immune system and induced the proliferation of PBMCs up to 8 times. Methanol extract of Agrimonia eupatoria showed antibacterial effects against gram-positive bacteria and the most antibacterial effect was on Bacillus subtilis at a concentration of 4 mg/ml and Staphylococcus aureus at concentration of 7 mg/ml. Conclusion: The Agrimonia eupatoria methanol extract showed stimulatory effects on the immune system and also antibacterial properties against certain gram-positive pathogenic bacteria. These finding indicate that Agrimonia eupatoria can be considered to use for immunodeficiency patients and moreover to control some bacterial infections.

Original languageFarsi
Pages (from-to)167-173
Number of pages8
JournalJournal of Medicinal Plants
Issue number61 + Suppl. 10
Publication statusPublished - 29 Jan 2017
Externally publishedYes

Bibliographical note

PDF only available in Farsi.


  • Agrimonia eupatoria
  • Antibacterial
  • Immunostimulator
  • PBMC


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