In-vitro methods for testing dermal absorption and penetration of toxic gases

Sharyn Gaskin, Dino Pisaniello, John Edwards, David Bromwich, Sue Reed, Michael Logan, Christina Baxter

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    8 Citations (Scopus)

    Abstract

    This technical note provides details of an experimental technique for in-vitro skin studies with atmospheric chemical challenge. There appear to be major evidence gaps in relation to dermal exposure of gases. We describe a modification of standard OECD protocols for an atmospheric delivery system which can be used to understand interaction of toxic gases and the skin. The system can be used to examine the mechanisms by which skin uptake occurs. Auxiliary components which allow for parameter variation such as temperature and relative humidity are also described. Methodology presented in this technical note uses examples of gas challenges (ammonia, chlorine) to illustrate its application to gases of differing physicochemical properties. This adapted protocol can be applied in the context of HAZMAT scenarios involving atmospheric toxic chemical release and dermal absorption potential under variable exposure conditions.

    Original languageEnglish
    Pages (from-to)70-72
    Number of pages3
    JournalToxicology Mechanisms and Methods
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - 2014

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  • Cite this

    Gaskin, S., Pisaniello, D., Edwards, J., Bromwich, D., Reed, S., Logan, M., & Baxter, C. (2014). In-vitro methods for testing dermal absorption and penetration of toxic gases. Toxicology Mechanisms and Methods, 24(1), 70-72. https://doi.org/10.3109/15376516.2013.859193