Incorporation of endothelial progenitor cells into mosaic pseudoislets

Daniella Penko, Daisy Mohanasundaram, Shaundeep Sen, Chris Drogemuller, Clare Mee, Claudine Bonder, P Coates, Claire Jessup

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Pancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in the longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both the survival and function of transplanted islets. Here, we describe for the first time the in vitro formation of rat mosaic pseudoislets comprised of pancreatic β-cells with interspersed vasculogenic EPC. Bone marrow-derived EPC displayed a similar phenotype to non-adherent EPC, recently described in the human and mouse. Mosaic pseudoislet formation was enhanced by the use of an embryoid body forming medium (BPEL) and a spin protocol. Mosaic pseudoislets maintained function in vitro and may represent an enhanced cell therapy delivery approach to enhance the survival and revascularization of transplanted islets.

    Original languageEnglish
    Pages (from-to)73-79
    Number of pages7
    JournalIslets
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - May 2011

    Keywords

    • Endothelial progenitor cells
    • Pancreatic islets
    • Pseudoislet
    • Revascularization
    • Transplantation

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