Increased endogenous dopamine prevents myopia in mice

E. G. Landis, M. A. Chrenek, R. Chakraborty, R. Strickland, M. Bergen, V. Yang, P. M. Iuvone, M. T. Pardue

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Experimental evidence suggests that dopamine (DA) modulates refractive eye growth. We evaluated whether increasing endogenous DA activity using pharmacological or genetic approaches decreased myopia susceptibility in mice. First, we assessed the effects of systemic L-3,4-dihydroxyphenylalanine (L-DOPA) injections on form deprivation myopia (FDM) in C57BL/6 J (WTC57) mice. WTC57 mice received daily systemic injections of L-DOPA (n = 11), L-DOPA + ascorbic acid (AA, n = 22), AA (n = 20), or Saline (n = 16). Second, we tested transgenic mice with increased or decreased expression of vesicular monoamine transporter 2 (VMAT2HI, n = 22; WTHI, n = 18; VMAT2LO, n = 18; or WTLO, n = 9) under normal and form deprivation conditions. VMAT2 packages DA into vesicles, affecting DA release. At post-natal day 28 (P28), monocular FD was induced in each genotype. Weekly measurements of refractive error, corneal curvature, and ocular biometry were performed until P42 or P49. WTC57 mice exposed to FD developed a significant myopic shift (treated-contralateral eye) in AA (−3.27 ± 0.73D) or saline (−3.71 ± 0.80D) treated groups that was significantly attenuated by L-DOPA (−0.73 ± 0.90D, p = 0.0002) or L-DOPA + AA (−0.11 ± 0.46D, p = 0.0103). The VMAT2LO mice, with under-expression of VMAT2, were most susceptible to FDM. VMAT2LO mice developed significant myopic shifts to FD after one week compared to VMAT2HI and WT mice (VMAT2LO: −5.48 ± 0.54D; VMAT2HI: −0.52 ± 0.92D, p < 0.05; WT: −2.13 ± 0.78D, p < 0.05; ungoggled control: −0.22 ± 0.24D, p < 0.001). These results indicate that endogenously increasing DA synthesis and release by genetic and pharmacological methods prevents FDM in mice.

Original languageEnglish
Article number107956
Number of pages9
JournalExperimental Eye Research
Volume193
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Dopamine
  • Form-deprivation
  • L-DOPA
  • Mouse
  • Myopia
  • Retina
  • VMAT2

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