Increasing DNA transfer efficiency by temporary inactivation of host restriction

Robert A. Edwards, R. Allen Helm, Stanley R. Maloy

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

E. coli and Salmonella typhimurium are widely used bacterial hosts for genetic manipulation of DNA from prokaryotes and eukaryotes. Introduction of foreign DNA by electroporation or transduction into E. coli and Salmonella is limited by host restriction of incoming DNA by the recipient cells. Here, we describe a simple method that temporarily inactivates host restriction, allowing high-frequency DNA transfer. This technique might be readily applied to a wide range of bacteria to increase DNA transfer between strains and species.

Original languageEnglish
Pages (from-to)892-900
Number of pages9
JournalBiotechniques
Volume26
Issue number5
DOIs
Publication statusPublished - May 1999
Externally publishedYes

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